女性肺腺癌CD44表达、DNA含量和凋亡率的相关性研究  

Correlation among expression of CD44, DNA content and apoptosis in female adenocarcinoma of the lung

作　　者：薛晓霞[1] 张莹[2] 蒋东华[1] 何钦成[1] 崔泽实[2] 周宝森[1] 

机构地区：[1]中国医科大学流行病学教研室,沈阳110001 [2]中国医科大学实验技术中心,沈阳110001

出　　处：《中国肺癌杂志》2007年第5期381-385,共5页Chinese Journal of Lung Cancer

基　　金：国家自然科学基金(No.30471493);美国中华医学基金(00-726)资助~~

摘　　要：背景与目的CD44基因蛋白是一种分布极为广泛的细胞表面跨膜糖蛋白,它的变异多样性表达与肿瘤的生长及转移密切相关。本研究的目的是探讨CD44的表达、DNA含量(DI)、增殖指数(PI)和细胞凋亡率在女性肺腺癌中的关系。方法应用流式细胞仪(FCM)测定61例女性肺腺癌及其癌旁肺组织和45例肺部良性病变组织中CD44的表达、DI、PI和细胞凋亡率。结果女性肺腺癌组DNA异倍体出现率为75.41%。肺癌组中CD44的表达、DI值和PI值明显高于癌旁对照组和良性对照组(P<0.01),肺癌组细胞凋亡率则显著低于癌旁对照组和良性对照组(P<0.01)。肺癌组中CD44的表达与DI值呈正相关(P<0.01),凋亡率与DI值呈负相关(P<0.01)。结论CD44表达、DNA含量、增殖活性和细胞凋亡等因素在女性肺腺癌的发生、发展和转移过程中具有重要的作用。Background and objective Cluster of differentiation 44 （CD44） is a family of transmembrane glycoproteins. As cell surface hyaluronate receptor, it has been found to be widely expressed in a variety of cells. The aim of this study is to assess the relationship among CD44 expression, DNA content, proliferation index （PI） and apoptosis in female adenocarcinoma of the lung. Methods The expression of CD44, DNA index （DI）, PI and apoptotic rate were studied in 61 cases of female adenocarcinoma of the lung, paracancerous tissues and 45 cases of benign lesions by flow cytometry. Results The percent of DNA aneuploidy was 75.41% in adenocarcinoma. The expression of CD44, DI and PI in adenocarcinoma were significantly higher than those in paracancerous and benign controls （P〈0.01）, however the apoptotic rate was obviously lower in adenocarcinoma than that in paracancerous and benign controls （P〈0.01）. There was a positive correlation between CD44 and DI （P〈0.01）, and a negative correlation between apoptosis and DI in adenocarcinoma （P〈0.01）. Conclusion The expression of CD44, DNA content, proliferation and apoptosis may play important regulating roles in oncogenesis, development and metastasis of female adenocarcinoma of the lung.

关 键 词：女性肺腺癌 流式细胞术 CD44 DNA 凋亡率 

分 类 号：R734.2[医药卫生—肿瘤]