肺癌患者与非肺癌人群CYP1A1基因MspⅠ位点多态性  

Polymorphism of CYP1A1 gene MspⅠ site in the lung cancer patients and non-lung cancer groups

作　　者：夏觅真[1] 薛绍礼[1] 金永堂[2] 孔云明[1] 王怡[1] 汪亚松[1] 侯勇[1] 

机构地区：[1]安徽医科大学医学生物工程教研室,安徽合肥230032 [2]浙江大学医学院,浙江杭州310058

出　　处：《现代医药卫生》2007年第21期3164-3165,共2页Journal of Modern Medicine & Health

基　　金：安徽省自然科学基金资助项目(NO.07021017);国家自然科学基金资助项目(NO.30471427)

摘　　要：目的:研究肺癌患者和正常人群CYP1A1基因MspⅠ位点的多态性。方法:应用聚合酶链反应-限制性片段长度多态技术对47例肺癌患者和94例非肺癌的基因型进行分析。结果:肺癌患者和非肺癌的CYP1A1基因A型(野生型)、B型(杂合型)和C型(突变型)在病例组的携带率分别为51.1%、40.4%和8.5%,对照组则分别为45.7%、39.4%和14.9%。经统计学检验,两组间差异无显著性(P>0.05),携带杂合型及纯合突变基因型(w/m,m/m)的个体患肺癌的危险性与CYP1A1基因MspⅠ多态性无相关性(OR=0.49,95%CI=0.15～1.56,P>0.05)。结论:CYP1A1基因MspⅠ多态性与肺癌发生无明显相关性。Objective：To study the Polymorphism of CYP1A1 gene Msp I site in the lung cancer patients and non-lung cancer groups.Methods：The PCR-restriction fragment length polymorphism （PCR-RFLP） technique was used to analyze the genotype of CYP1A1 gene Msp I site in 47 lung cancer patients and 94 controls,among them there was no blood relatinoship each other.Results：The genotype frequency of CYP1A1 gene Msp Ⅰ site showed that the wild-type, heterozygote and homozygoas variants were 51.1% ,40.4%, 8.5% and 45.7% ,39.4%, 14.9% respectively distribution in the lung cancer patients and the controls. Statistic analysis suggested that there was no significant difference between two groups.Conclusion：The genotype frequency distribution of CYP1A1 gene MspⅠ site did not exhibit the obvious difference between the lung cancer patients and the controls.

关 键 词：细胞色素P450 基因多态性 肺癌 

分 类 号：R73[医药卫生—肿瘤]