7Gyγ射线照射后4h小鼠骨髓差异表达基因的初步研究  

Preliminary research on differentially expressed genes in mouse bone marrow 4h after 7Gy γ-ray radiation in vivo

作　　者：饶亚岚[1] 陈肖华[1] 王琳[1] 廖于峰[1] 董波[1] 丛悦[1] 张军权[1] 高玲[1] 李峰生[1] 毛秉智[1] 

机构地区：[1]北京军事医学科学院放射与辐射医学研究所一室,100850

出　　处：《国际放射医学核医学杂志》2007年第4期245-247,256,共4页International Journal of Radiation Medicine and Nuclear Medicine

基　　金：国家自然科学基金项目（30400119）

摘　　要：目的为探讨急性放射病骨髓损伤的分子机制，研究整体照射条件下，辐射前后骨髓基因表达的变化。方法运用抑制消减杂交、cDNA阵列杂交及Northern杂交等方法，筛选C57BL／6J小鼠受7Gy^60Coγ射线照射后4h骨髓组织差异表达基因。结果筛选到一系列辐射后可能表达升高的基因，其功能涉及细胞周期调控、抗氧化、DNA损伤修复和造血免疫，实验确证CDKN1A及S100A8基因的差异表达。结论辐射后差异表达基因的功能说明骨髓受辐射后即发生哺乳细胞普遍发生的辐射效应（如DNA损伤、细胞周期阻滞、过氧化反应），同时骨髓的造血免疫功能及神经内分泌调节发生改变。Objective To explore the molecular mechanism of bone marrow injury of acute radiation syndrome, gene expression of bone marrow in mice after whole body irradiation was studied. Methods The molecular biology technology including suppression subtraetive hybridization, eDNA array hybridization and Northern hybridization were used to study differential gene expression of roRNA level in bone marrow of C57BL/6J mouse 4h after 7Gy^60Coγ-ray radiation in vivo. Results A series possibly induced genes after irradiation were obtained. The function of these genes related to cell cycle control, anti-oxidation, DNA repair, and heroatopoiesis-iroroune response. The differential expression of CDKN 1A and S100A8 gene were validated by further experiment. Conclusions The function of those differentially expressed genes after irradiation illuminated that radiation effects, such as DNA damage, cell cycle arrest and oxidation reaction which often happened in mammalian cells, also took place in bone marrow cells after irradiation. Furthermore, the heroatopoiesis-iroroune function and the neumirorounoendoerine regulation in bone marrow were also changed.

关 键 词：骨髓 辐射损伤 基因表达谱 小鼠 近交C57BL 

分 类 号：R73[医药卫生—肿瘤]