FBN2基因与肺癌细胞关系的初步探讨  

The primary study about the relationship of FBN2 gene and lung cancer cell lines

作　　者：陈虹[1] 

机构地区：[1]重庆医科大学附属第一医院呼吸内科,重庆400016

出　　处：《重庆医科大学学报》2007年第11期1175-1177,1195,共4页Journal of Chongqing Medical University

基　　金：重庆医科大学第一附属医院科研启动基金;重庆医科大学科研启动基金(QD200502);重庆市科委课题(2006BB5033)

摘　　要：目的:探讨FBN2基因在肺癌细胞中的表达及其受甲基化的调控。方法:培养肺癌细胞株HCC366、H1299、H2195和人正常支气管上皮细胞(NHBEC),并用5-Aza-cdR干预。用逆转录方法观察FBN2基因的表达,并用MSP检测是否存在FBN2的异常甲基化状态。结果:①FBN2基因在HCC366、H1299不表达,但在NHBEC、H2195中表达。②经过5-Aza-cdR处理后,FBN2基因在HCC366、H1299可以重新表达。③HCC366、H1299细胞存在FBN2基因的异常甲基化表现现象。结论:FBN2基因在HCC366、H1299细胞中不表达,但5-Aza-cdR能使其重新表达。FBN2基因沉默可能是受启动子异常甲基化调控的影响。Objective：To study the gene expression and aberrant methylation of FBN2 on lung cancer cell lines. Methods： Three lung cancer cell lines HCC366, H1299 and H2195 were cultured, mRNA was analysed by RT-PCR and methylation status was also investigated by methylation specific PCR. The loss of FBN2 expression cell lines were treated with the demethylating agent, 5-aza-2＇- deoxycytid-ine （5-aza-cdR）. Results：The expression of FBN2 was detected in NHBEC and H2195,whereas it was not detected in HCC366 and H1299 which showed aberrant methylation of FBN2. FBN2 expression was restored after treatment with 5-aza-cdR. Conclusion：Methylation and silencing of FBN2 in tumor cells may play an important role in carcinogenesis of lung cancer.

关 键 词：FBN2 肺癌细胞 甲基化 5-Aza—cdR 

分 类 号：R734.2[医药卫生—肿瘤]