SOD对表没食子儿茶素没食子酸酯抗肿瘤活性及稳定性的影响  

Effect of SOD on Anti-cancer Activity and Stability of Epigallocatechin-3-gallate

作　　者：张斌[1] 夏艳秋[1] 金礼吉[1] 徐永平[1] 

机构地区：[1]大连理工大学环境与生命学院生物科学与工程系,辽宁大连116024

出　　处：《茶叶科学》2007年第4期293-298,共6页Journal of Tea Science

基　　金：国家杰出青年基金(30125034)

摘　　要：采用MTT方法研究表没食子儿茶素没食子酸酯(EGCG)对人结肠癌细胞HT-29、人乳腺癌细胞MCF-7和人宫颈癌细胞Hela生长的影响。通过检测培养基中乳酸脱氢酶的活力研究EGCG对三种细胞的细胞毒作用,用流式细胞仪检测EGCG对HT-29的凋亡影响,用HPLC方法分析EGCG在培养基DMEM/F-12中的稳定性。以上研究均设加入SOD和不加SOD两个对照组。研究表明:与没有SOD存在条件下相比,加入SOD后,细胞存活率下降,培养基中LDH活性增强,HT-29细胞凋亡率上升;EGCG在培养基中的稳定性增强。Effects of epigallocatechin-3-gallate （EGCG） on the growth of human colonic cancer cells HT-29, human breast cancer MCF-7 and human cervical cancer Hela were investigated by MTC assay. Cytotoxicity of EGCG on these cells was detected by determining the activity of LDH in culture medium. Induction of apoptosis in HT-29 cell after the EGCG treatment was analyzed by flow cytometry. Stability of EGCG in DMEM/F-12 was determined by HPLC and all studies above were compared in the presence or absence of SOD. Investigation showed that cells survival percentage deceased and activity of LDH in the culture medium increased after adding SOD. The apoptotic rate of HT-29 and the stability of EGCG in culture increased in the presence of SOD comparing to absence of SOD.

关 键 词：EGCG SOD HT-29细胞 

分 类 号：R73-3[医药卫生—肿瘤]