血管紧张素Ⅱ上调血管平滑肌细胞富含半胱氨酸蛋白61的表达  

Angiotensin Ⅱ Up-Regulates Vascular Smooth Muscle Cells Cysteine-Rich 61 Expression Via Extracellular Signal Regulated Kinase 1/2

作　　者：付鹏[1] 牛铁生[1] 孙英贤[1] 

机构地区：[1]中国医科大学附属盛京医院心内科,辽宁省沈阳市110004

出　　处：《中国动脉硬化杂志》2007年第6期431-434,共4页Chinese Journal of Arteriosclerosis

摘　　要：目的探讨血管紧张素Ⅱ对培养鼠血管平滑肌细胞富含半胱氨酸蛋白61的影响及其可能的转导通路。方法组织贴块法培养血管平滑肌细胞,血管紧张素Ⅱ处理平滑肌细胞15min、30min、60min、180min,PD98059在血管紧张素Ⅱ刺激细胞前1h加到培养基中,用逆转录聚合酶链反应和Westernblotting检测分析富含半胱氨酸蛋白61的表达。结果逆转录聚合酶链反应分析显示用1μmol/L血管紧张素Ⅱ刺激细胞培养15min、30min富含半胱氨酸蛋白61的表达与对照组相比明显增加;同时细胞外信号调节激酶1/2活性高于对照组(P<0.01),60min时仍有表达,180min时减弱;细胞外信号调节激酶1/2特异性抑制剂PD98059(20μmol/L)能阻断血管紧张素Ⅱ上调富含半胱氨酸蛋白61的表达并抑制增加的磷酸化细胞外信号调节激酶1/2活性。结论血管紧张素Ⅱ通过细胞外信号调节激酶1/2促进血管平滑肌细胞上调富含半胱氨酸蛋白61表达,这可能是血管紧张素Ⅱ促血管平滑肌细胞增殖的一个重要机制。Aim To investigate the effect of angiotensin Ⅱ（AngⅡ） on cysteine-rich 61（Cyr61） expression and its transduction access in cultured rat vascular smooth muscle cells（VSMC）.Methods VSMC were cultured in sticking mass methods.VSMC were treated with AngⅡfor 15 min,30 min,60 min,180 min,which were pretreated with PD98059 for 60 min before AngⅡ treatment.The Cyr61 expression in VSMC were characterized by reverse transcription polymerase chain reaction（RT-PCR） and Western blotting.Results RT-PCR analysis revealed that Cyr61 increased in cells treated with 1 μmol/L AngⅡ for 15,30 min compared with control（P〈0.01） and still expressed at 60 min and attenuated at 180 min.Phosphorylated extracellular signal regulated kinase（P-ERK） 1/2 activity increased in cells treated with 1 μmol/L AngⅡ for 30 min compared with control（P〈0.01）,Cyr61 expression and P-ERK1/2 activity were inhibited by the presence of 20 μmol/L PD98059,a specific inhibitor of ERK1/2.Conclusions AngⅡup-regulates Cyr61 expression in cultured rat VSMC via ERK1/2.This may be a significant mechanism for VSMC proliferation by AngⅡ.

关 键 词：病理学与病理生理学 血管紧张素Ⅱ 血管平滑肌细胞 富含半胱氨酸蛋白61 细胞外信号调节激酶 丝裂原活化蛋白激酶 

分 类 号：R63[医药卫生—外科学]