脑部特异表达SV40Tag转基因动物模型的建立  

Establishment of transgenic mouse model expressing SV40Tag in brain

作　　者：冯洁[1] 孙强[2] 邢华[3] 高诚 李厚达[3] 

机构地区：[1]上海实验动物研究中心,上海200032 [2]华东师范大学,上海200062 [3]扬州大学兽医学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2007年第3期9-12,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家"十五"科技攻关项目(2001BA70113)

摘　　要：将猿猴病毒抗原蛋白(SV40Tag)片段克隆进脑部特异表达载体pMM279中,通过显微注射法制作转基因小鼠。采用PCR和Southern blotting检测目的基因整合,并通过RT-PCR检测目的基因的表达。结果表明:共出生29只仔鼠,经PCR检测出3只阳性,Southern blotting检测出2只阳性。RT-PCR检测到SV40Tag仅在前脑部皮层和海马表达。成功获得的脑部特异表达SV40Tag转基因小鼠模型,为SV40Tag的致病机制及脑肿瘤的治疗等研究提供工具。SV40Tag was subcloned into pMM279 and the recombinant vector was used to generate transgenic mice by microinjection. Transgene integration was detected by PCR and southern blot, and tissue specificity of the transgene expression was confirmed by RT-PCR. A total of 29 mice were obtained, of which 3 carried the transgene. The expression of SV40Tag was detected only in cortex and hippocampus. In conclusion, we have established a transgenic mouse model expressing SV40Tag in brain. It can be used for studying the nosogenesis of SV40Tag and cure of brain tumor.

关 键 词：脑肿瘤 猿猴病毒抗原蛋白 转基因 

分 类 号：R739.4[医药卫生—肿瘤] Q786[医药卫生—临床医学]