鸡GARS-AIRS-GART基因5′侧翼序列多态性与鸡肉IMP含量的关联分析  

Single nucleotide polymorphism in 5′ flanking of GARS-AIRS-GART gene in chickens and its relationship with IMP contents of breast muscles

作　　者：束婧婷[1] 张学余[2] 季从亮[1] 吴信生[1] 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]中国农业科学院家禽研究所,江苏扬州225003

出　　处：《扬州大学学报（农业与生命科学版）》2007年第3期27-30,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家高技术研究发展计划项目(863-2006AA10Z1D8);国家自然科学基金资助项目(30170673)

摘　　要：以鸡GARS-AIRS-GART基因为候选基因,根据5′侧翼序列设计引物,用PCR-SSCP方法对隐性白羽鸡、丝羽乌骨鸡、萧山鸡、白耳鸡及藏鸡进行单核苷酸多态性分析,并检测到多态性,表现为3种基因型,对2种纯合子进行直接测序,结果发现-179位点发生了突变,由C突变为T。对3种基因型的肌肉肌苷酸(IMP)含量采用最小二乘法分析,结果显示TT型(突变型)个体的肌肉IMP含量极显著高于CT型、CC型个体,CT型个体稍高于CC型,但差异不显著,初步推测该位点可能与肌肉IMP含量有关,可作为肌肉IMP含量的辅助选择标记。This study was designed to investigate the single nucleotide polymorphism （SNP） of GARS-AIRS-GART gene in various breeds, including Recessive White chickens, Silkies chickens, Xiaoshan chickens, Baier chickens and r Tibetan chickens. The primers for 5′ flanking in GARS-AIRS-GART gene were designed from the database of chicken genomic sequence and the SNPs were detected by PCR-SSCP and DNA sequencing. Three genotypes were found among individuals within all breeds and one SNP （C→T at -179） was found. However, the amino acid was not changed because it was a silence mutation. The least square analysis showed that TT genotype birds had significant higher （P〈0.01） IMP content than TC and CC genotype birds, TC genotype birds also had a little higher IMP content than CC genotype birds, but the difference was not significant. We concluded that there may be a relationship between this SNP site and IMP content in chickens.

关 键 词：鸡 GARS—AIRS—GART基因 肌苷酸 单核苷酸多态性 

分 类 号：S831.2[农业科学—畜牧学]