三氧化二砷诱导9L胶质瘤细胞凋亡的实验研究  

Experimental Study of 9L Glioma Cells Apoptosis Induced by Arsenic Trioxide

作　　者：邢立举[1] 唐海涛[1] 贾德燕[1] 郄福忠[1] 张良玉[2] 鲁春鹤[1] 王玉春[1] 

机构地区：[1]大庆油田总医院神经外科,黑龙江大庆163001 [2]大庆油田总医院肿瘤科,黑龙江大庆163001

出　　处：《中国临床神经外科杂志》2007年第10期614-616,共3页Chinese Journal of Clinical Neurosurgery

摘　　要：目的研究三氧化二砷(As2O3)对鼠胶质瘤生长的影响及其机制。方法应用不同浓度As2O3,分别处理9L胶质瘤细胞株不同时间,采用四甲基噻唑蓝比色(MTT)法观察As2O3对9L胶质瘤细胞生长的影响,以透射电镜、Hoechst 33342/PI双染荧光及流式细胞仪检测9L胶质瘤细胞凋亡。结果不同浓度的As2O3均可显著抑制9L胶质瘤细胞株的生长。As2O3作用可引起9L胶质瘤细胞的凋亡,且随As2O3浓度的增大和作用时间的延长,9L胶质瘤细胞的凋亡率明显上升。结论As2O3在体外可显著抑制9L胶质瘤细胞生长,其机制与诱导肿瘤细胞凋亡有关,且其凋亡率随As2O3作用的时间延长和剂量的增加而增加。Objective To study curative effect of arsenic trioxide（As2O3）on 9L glioma cells in vitro and its mechanism. Methods Rats＇ 9L glioma cells were cultured for different time in the media containing different concentrations of As2O3. The effects of As2O3 on 9L glioma cell growth was observed by methyl thiazolyl tetrazolium （MTT） method. Morphological Change in 9L glioma cells was observed by transmission electron microscope and Hoechst 33342/ PI staining. The apoptosis rate of 9L cells was detected by Annexin -v-FITC/PI assay. Results As2O3 at the concentration of 0.5～8.0 μmol/L significantly inhibited the growth of 9L glioma cells compared to the control group. Electron microscope and Hoechst 33342/ PI staining revealed that there was significant apoptosis of 9L glioma cells cultured in the media containing As2O3. Apoptosis rates of 9L glioma cells was significantly elevated with the increases in action time and concentration of As2O3. Conclusion As2O3 can significantly inhibit the growth of 9L glioma cell in vitro by inducing apoptosis of 9L cells in time-and dose-dependent way.

关 键 词：三氧化二砷 9L细胞 细胞凋亡 

分 类 号：R739.41[医药卫生—肿瘤] R73-361[医药卫生—临床医学]