聚ADP核糖聚合酶1在人类乳腺癌中的表达及其与临床预后相关因子的关系  

Expression of Poly ADP-Ribose Polymerase-1 in Human Breast Cancer Tissue and Its Relationship with Prognosis Related Factors

作　　者：孔萌萌[1] 陆东东[1] 陈宇星[1] 

机构地区：[1]同济大学医学部生命科学与技术学院,上海市200092

出　　处：《中国康复理论与实践》2007年第10期930-932,共3页Chinese Journal of Rehabilitation Theory and Practice

摘　　要：目的研究聚ADP核糖聚合酶1(PARP-1)在人类乳腺癌中的表达及其与患者临床预后相关因子间的相关性。方法采用RT-PCR及免疫荧光染色技术,检测66例手术切除的人类乳腺癌组织及癌旁组织中PARP-1的表达,并分析乳腺癌患者的康复相关因子与PARP-1表达间的关系。结果与癌旁组织相比,94.9%(62/66)的癌组织中PARP-1 mRNA表达上调,90.9%(60/66)的癌组织中PARP-1蛋白表达上调。临床分期越晚、肿瘤分化程度越差,PARP-1的标记指数(LI)、表达指数(EI)越大;单纯癌、浸润癌患者中PARP-1的LI、EI值较大;乳腺癌转移患者的PARP-1的LI、EI值显著高于非转移患者。结论PARP-1基因在转录和翻译水平上的过表达与乳腺癌的分期、病理分级、病理类型、肿瘤转移等临床预后相关因子有关。Objective To investigate the expression of poly ADP-ribose polymerase-1 （PARP-1） in human breast cancer tissue and its relationship with patient＇s prognosis related factors. Methods The expression of PARP-1 in breast cancer tissues and their adjacent non-cancerous tissues from resection in 66 operated cases were detected using RT-PCR and immunofluorecence staining （IFS）. The relationship between the expression of PARP-1 and the prognosis related factors was analyzed. Results Compared with that of the adjacent non-cancerous tissues, the expression of PARP-1 mRNA increased in 94.9% （62/66） patients, while the expression of PARP-1 protein increased in 90.9% （60/66） ones. The label index （LI） and the expression index （EI） of PARP-1 gene increased especially in the cases in later clinical stage, with poor tumor differentiation grade, of pure breast carcinomas or infiltrative breast carcinomas and of metastasis. Conclusion The expression of PARP-1 in human breast cancer tissue on the level of transcription and translation increases, which may be positively related with the patient＇s prognosis.

关 键 词：聚ADP核糖聚合酶1(PARP-1) 乳腺癌 预后 

分 类 号：R735.3[医药卫生—肿瘤]