三氧化二砷在体外抑制结肠癌细胞的研究(英文)  

A study on arsenic trioxide inhibiting in vitro growth of human colon cancer cells

作　　者：梁德森[1] 田茂霖[2] 吕海涛[2] 汪大伟[2] 李正天[2] 

机构地区：[1]华中科技大学同济医学院同济医院普外科,湖北武汉430030 [2]哈尔滨医科大学第一临床医学院普外科,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》2007年第5期444-448,共5页Journal of Harbin Medical University

摘　　要：目的在体外观察三氧化二砷(As2O3)对结肠癌细胞系HT-29的抑制作用,并探讨其作用机制,为其是否能作为结肠肿瘤的化疗药物提供理论基础。方法将不同浓度As2O3作用于HT-29细胞不同时间后,用MTT检测细胞抑制率,光镜及透射电镜观察细胞形态学和超微结构的变化,通过流式细胞仪及共聚焦显微镜分析凋亡及自噬。结果4.0μmol/L As2O3作用72h可明显抑制HT-29细胞增殖及诱发其凋亡,且抑制率与药物作用时间及浓度成正比。在凋亡早期细胞形态学变化在光镜下可见细胞缩小变圆,在电镜上可见细胞质浓缩、核固缩及自噬溶酶体。4.0μmol/L As2O3作用72h后HT-29细胞凋亡率为25%。结论As2O3能明显抑制结肠癌细胞系HT-29的增殖,其作用机制与引起凋亡及自噬密切相关。Objective To observe the effect of arsenic trioxide on the proliferation of human colon cancer cells HT-29 in vitro and investigate its mechanism. Methods HT-29 cells （a human colon cancer cell line） were treated with arsenic trioxide in different concentrations. The inhibition on the proliferation of HT-29 cells was estimated by MTT-assay. Morphological changes were observed under light microscope and electron microscope. Apoptosis and autophagy were analyzed by flow cytometer and confocal microscopy. Results When treated with 4.0μmol/L arsenic trioxide for 72h,the proliferation of HT-29 cells was obviously inhibited and the cell underwent conspicuous apoptosis. The inhibition on the proliferation depended on the exposure time and dose. The cells showed morphological changes at the early stage of apoptosis under the light microscope：the shrunk and round cells;condensed cytoplasma and pycnosis nucleus and autophasosomes could be found under the transmission electron microscope. The rate of the apoptotic cells was 25 % after intervention with low concentration of arsenic trioxide （4.0μmol/L） for 72h. Conclusion Arsenic trioxide could significantly inhibit the proliferation of HT-29 cells, and the proliferation inhibition is closely related to cell apoptosis and autophagy.

关 键 词：结肠癌细胞 三氧化二砷 凋亡 自噬 

分 类 号：R735.35[医药卫生—肿瘤]