化疗对胃癌原发灶和淋巴结转移灶癌细胞凋亡的影响  

Effect of preoperative chemotherapy on apoptosis of primary locus and lymph node metastasis of gastric cancer

作　　者：崔滨滨[1] 邢军[1] 刘彦龙[1] 

机构地区：[1]哈尔滨医科大学第三临床医学院腹外科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2007年第5期449-451,共3页Journal of Harbin Medical University

基　　金：黑龙江省青年基金项目(Q00C007)

摘　　要：目的探讨术前化疗对胃癌原发灶和淋巴结转移灶癌细胞凋亡的影响。方法40例患者行胃癌根治性切除术,均经病理学确诊为胃癌伴淋巴结转移,其中20例行术前化疗,采用免疫组化ABC法检测胃癌原发灶和淋巴转移灶p53、bcl-2及CD95基因的表达,并用原位末端脱氧核苷转移酶标记法(TUNEL)检测细胞凋亡情况。结果术前化疗对胃癌原发灶和淋巴结转移灶癌同样有效;术前化疗可以抑制bcl-2表达,明显增强胃癌淋巴结转移灶癌细胞p53和CD95的表达,并与细胞凋亡呈正相关。胃癌原发灶和淋巴转移灶p53、CD95和bcl-2表达差异无统计学意义(P>0.05),两者细胞凋亡差异亦无统计学意义(P>0.05)。结论术前化疗影响p53,CD95和bcl-2的表达,进而诱导胃癌细胞的凋亡,对胃癌原发灶和淋巴结转移灶的影响作用相同,对于改善胃癌根治术有一定的作用。Objective To study the effect of preoperative chemotherapy on apoptosis of primary locus and lymph node metastasis of gastric cancer. Methods Forty patients with gastric cancer and lymph node metastasis underwent curative resection, twenty of them received the preoperative gastric arterial chemotherapy, who constituted the treatment group, and the rest of the patients were treated as the control group. The expressions of p53, CD95 and bcl-2 were detected by immunohistochemistry and apoptosis in the lymph node metastasis was detected by in situ terminal transferase-mediated dUTP nick end labeling （TUNEL）. Results The expression intensity of p53 and CD95 in lymph node metastasis of the treatment group increased mere significantly than that of control group, whereas-the expression intensity of bcl-2 decreased in treatment group. There was a significantly positive correlation between the expressions of p53 and CD95 and the apoptosis. And there was no significance between lymphode group and primary locus group. Conclusion Preoperative chemotherapy may affect the expressions of p53, CD95 and bcl-2 and induce the apoptosis of lymph node metastasis. It may be helpful to improve the effect of curative resection of gastric cancer.

关 键 词：胃癌 化疗 p53 CD95 bcl-2 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]