JAK-STAT信号通路在创伤性深静脉血栓形成中作用的实验研究  

To Study Influence of JAK-STAT Signaling Pathway in Traumatic Deep Vein Thrombosis

作　　者：莫建文[1] 黄河[1] 张春强[1] 王兵[1] 何飞[1] 殷亮[1] 赵智[1] 唐锡章[1] 周兆文[1] 赵学凌[1] 李世和[1] 

机构地区：[1]昆明医学院第一附属医院骨科,云南昆明650032

出　　处：《中国误诊学杂志》2007年第26期6212-6214,共3页Chinese Journal of Misdiagnostics

基　　金：云南省科技厅重大攻关项目(编号:2005NG09);云南省自然科学基金面上项目(编号:2005C0071M)

摘　　要：目的:探讨Jak蛋白酪氨酸激酶-信号转导子和转录激活子信号通路在创伤性深静脉血栓形成中的作用。方法:将150只SD大鼠随机分为正常对照(A组)和模型组。模型组根据造模后的不同生物学状态再分为7组:创伤即刻(B组)、血栓形成前期(C组)、高峰期血栓形成(D组)、高峰期血栓不形成(H组)、血栓消退期(E组)、血栓不消退(F组)和血栓不形成(G组),在相应时相点无创切取股静脉血管组织,随后抽取各组大鼠总RNA,用Genechip Rat Genome430 2.0芯片测定股静脉RNA表达,并分析Jak蛋白酪氨酸激酶-信号转导子和转录激活子信号通路基因表达变化情况。结果:Jak蛋白酪氨酸激酶-信号转导子和转录激活子信号通路中Cytokine R、STAT、PI3K、AKT、SOCS、CycD等关键基因均呈下调,调控着细胞凋亡、细胞周期等。结论:Jak蛋白酪氨酸激酶-信号转导子和转录激活子信号通路可能是调控血栓的生物学状态的重要信号通路之一。Objective：To study the significance of janus protein tyrosine kinase-signal transducer and activator of transcription signaling pathway in traumatic deep vein thrombosis.Methods：150 rats were divided into control（group A） and experience groups.The experience group was divided into 7 groups according to biological states：the post-traumatic instant（group B）,the initial period of thrombosis（group C）,the crest-time of thrombosis（group D）,without thrombosis at the crest-time（group H）,thrombi solution（group E）,thrombi insolution（group F）and no thrombosis（group G） groups.To incise the femoral vein in corresponding time,to adopt to Trizol onestep method for total RNA extraction of femoral vein.After applying Genechip Rat genome 430 2.0 genechips to detect the RNAs expressions,the genes related to janus protein tyrosine kinasesignal transducer and activator of transcription signaling pathway were selected through genechip data analyasis.Results：The key genes related to janus protein tyrosine kinase-signal transducer and activator of transcription signaling pathway of Cytokine R,STAT,PI3K,AKT,SOCS,CycD were down regulation,which controled the apoptosis and cell cycle of the vascular endothelial cell.Conclusion：Janus protein tyrosine kinase-signal transducer and activator of transcription signaling pathway may be one of the most important signaling pathway to control the biological states of thrombi.

关 键 词：静脉血栓形成/病理生理学 蛋白质酪氨酸激酶/生理学 信号传导 大鼠 Sprague-Dawley 随机分配 动物 

分 类 号：R364.15[医药卫生—病理学]