利用mtDNA控制区序列分析斑点叉尾鮰的遗传多样性  

Analysis of Genetic Diversity of Channel Catfish(Ictalurus punctatus) by mtDNA Sequence

作　　者：葛陇利[1] 赫崇波[2] 高祥刚[2] 陈姝君[3] 丛林林[3] 曹洁[1] 

机构地区：[1]大连水产学院生命科学与技术学院,辽宁大连116023 [2]辽宁省海洋水产科学研究院辽宁省海洋水产分子生物学重点实验室辽宁,大连116023 [3]辽宁师范大学生命学院,辽宁大连116029

出　　处：《水产科学》2007年第10期547-550,共4页Fisheries Science

基　　金：国家自然科学基金资助项目(30571410)

摘　　要：利用m tDNA控制区序列对1984、1997年引进的斑点叉尾鮰群体遗传多态性进行了分析。通过PCR产物测序法,测定了40尾斑点叉尾鮰线粒体DNA的一段包括部分Cytb基因、tRNAThr、tRNAPro、D-loop控制区和部分tRNAThe长度为1256 bp的核苷酸序列,经过比对分析得到7个序列单元型。7个单元型中共有13个碱基转换位点,全部发生在控制区,其序列相似度平均为99.58%。群体总的序列单元型多态性比例为17.5%,其中1997年群体的单元型多态性比例为35%,是1984年群体单元型多态性比例(15.0%)的2倍多。初步表明1984年引进的群体遗传多样性较低。The genetic diversity of two populations of channel catfish（Ictalurus punctatus）（introduced in 1984 and 1999 from the USA） was analyzed using mtDNA control region.The sequences of partial Cytb,tRNA^Thr,tRNA^Pro,D-loop control region and partial tRNA^The were sequenced using PCR-based sequencing method from 40 individuals consisting of 1256 bp.Seven haplotypes were obtained from these 40 sequences whose similarity was 99.58% and there were 13 variation sites of transition in the control region and no variation in other genes.Percentage of haplotypes was 17.5% in the overall population,35% in the population 1997,and 15.0% in the population 1984,indicating that the genetic diversity of population 1984 was declining.

关 键 词：斑点叉尾鮰 线粒体DNA 单元型 遗传多样性 

分 类 号：Q78[生物学—分子生物学]