骨髓间充质干细胞视网膜下移植对光损伤SD大鼠光感受器细胞凋亡的影响  

Effects of bone marrow mesenchymal stem cells subretinal transplantation upon photoreceptor apoptosis in light damaged SD rats.

作　　者：张钰[1] 张弛[1] 王薇[1] 

机构地区：[1]北京大学第三医院北京大学眼科中心,北京100083

出　　处：《眼外伤职业眼病杂志》2007年第10期741-745,共5页Journal of Injuries and Occupational Diseases of the Eye with Ophthalmic Surgeries

摘　　要：目的研究骨髓间充质干细胞(BMSC)视网膜下移植对光损伤SD大鼠光感受器细胞凋亡的影响及可能的机制。方法24只SD大鼠分为正常组,光损伤组,PBS注射组,BMSC视网膜下移植组。除正常组外大鼠接受绿光照射24h,光照后10 d手术,术后14 d或光照后24 d眼球摘除,制作冰冻切片,行HE染色,免疫荧光和Tunel凋亡检测。对各组外核层层数及凋亡细胞百分数行方差分析。结果BMSC移植组与PBS注射组及光损伤组相比,外核层层数显著增加,凋亡细胞百分比显著减少。BMSC在视网膜下腔表达Nestin,不表达MAP2、Rhodopsin、Calretin in和CD11b;表达bFGF,不表达BDNF和CNTF。结论BMSC视网膜下移植可抑制光损伤大鼠光感受器的凋亡,可能是通过其分泌的bFGF发挥作用。Objective To investigate the effect and possible mechanism of bone marrow mesenchymal stem cells（BMSC） subretinal transplantation upon photoreceptor apoptosis in light damaged SD rats. Methods 24 SD rats were divided into normal group,light damage group,PBS injection group and BMSC transplantation group. Except for normal group,SD rats were exposed to green continuous light for 24 hours.Eyes underwent operation 10 days after exposure,and were enucleated 14 days postoperatively or 24 days after exposure.Eyes were prepared as cryosections,which were performed HE stain,immunofluorescence and Tunel apoptotic detection.Variance analysis were done to compare rows of ONL cells and percents of apoptotic ONL cells among groups. Results Compared with PBS injection group and light damage group,rows of ONL cells significantly increased and percents of apoptotic ONL cells significantly decreased in BMSC transplantation group.BMSC in subretinal cavity expressed Nestin,never expressed MAP2、Rhodopsin、Calretinin and CD11b.BMSC expressed bFGF,never expressed BDNF and CNTF. Conclusions BMSC subretinal transplantation inhibited photoreceptor apoptosis in light damaged rats,that was possibly due to the trophic effect of bFGF secreted by BMSC.

关 键 词：骨髓间充质干细胞 视网膜下移植 光损伤:碱性成纤维细胞生长因子 

分 类 号：R779.1[医药卫生—眼科]