薄层色谱扫描法测定乙氧硬化醇  

Scanning TLC Determination of Lauromacrogol

作　　者：张嘉[1] 李多伟[1] 孙诗清[1] 令狐昱慰[1] 

机构地区：[1]西北大学生命科学学院,西安710069

出　　处：《理化检验（化学分册）》2007年第10期868-869,共2页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：应用薄层色谱法测定制剂中乙氧硬化醇的含量。测定中采用CS-930型双波长薄层色谱扫描仪，将由硅胶G及羧甲基纤维素钠溶液制成的浆状液均匀涂布于色谱板上，涂布薄层的大小为10cm×10cm。晾干后，置于105℃控温的烘箱中活化30min，用毛细管定量点样管将试样及标准的乙氧硬化醇溶液点于薄层板上，每点溶液的体积为5μL。经展开和晾干后，将试样点及标准点置于碘蒸气中显色。显色后立即取出，密封，并放置30min后按试验方法进行单波长扫描，选择显色物的吸收峰430nm作为测定波长。点面积的积分值和点浓度在10-50μg／点之间呈线性关系，连续测定6次，计算测得结果的RSD值为1．28％，5次测定所得的回收率平均值为100．3％。Thin layer chromatography （TLC） was applied to the determination of lauromacrogol in its preparations. A dual-wavelength thin layer chromatographic scanner （CS-930） was used in the determination. A slurry of silica gel G and solution of sodium carboxy methyl cellulose （CMC） was made and spread evenly on the plate in the size of 10 cm×10 cm. After being dried, it was activated for 30 min at 105 ℃ in an electric oven. Solutions of samples and standard lauromacrogol in ethanol were spotted （5 μL per spot） on the thin layer plate using quantitative micro-capillary spot-sampler. After developing and drying, the spots （both of sample and standard） were colorized in iodine vapor, and then taken out and sealed hermetically. After staying for 30 min, the spots were scanned as described. The measuring wavelength was taken at the absorption maxima of 430 nm, Linear relationship between the integral of spot area and concentration of lauromacrogol in the spot was kept in the range from 10 to 50μg per spot. Value of RSD＇s （n= 6） found was 1.28%. Value of average recovery of 5 determinations was found as 100. 3%.

关 键 词：薄层色谱法 单波长扫描 乙氧硬化醇 

分 类 号：O657.37[理学—分析化学]