Sca-1^+ Lewis肺癌细胞成瘤实验的研究  

Tumorigenicity of Sca-1 Positive Cells from Lewis Lung Carcinoma Cell Line in C57BL/6 Mice

作　　者：敖绪军[1] 钱莘[1] 安江宏[1] 孙建国[1] 陈正堂[1] 

机构地区：[1]第三军医大学新桥医院全军肿瘤研究所,重庆400037

出　　处：《重庆医学》2007年第20期2044-2045,2048,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(30672076)。

摘　　要：目的探讨小鼠Lewis肺癌(Lewis lung carcinoma,LLC)细胞株中Sca-1+细胞的成瘤特性。方法以Sca-1作为磁珠分选细胞的表面标志,从LLC细胞中分离Sca-1+细胞,流式细胞仪鉴定分离的纯度。将分选的Sca-1+细胞、Sca-1-细胞及未分选细胞分别以1×106、5×105、1×105、5×104、2×104密度接种于C57BL/6小鼠腋窝下,观察成瘤时间,4周后处死小鼠,统计成瘤率、平均瘤重及体积。结果分选前Sca-1阳性细胞百分比为(8.06±1.03)%,分选后Sca-1阳性细胞百分比为(93.92±1.07)%。Sca-1+细胞组在2×104个细胞时可以成瘤,而Sca-1-细胞组最少需要5×105个细胞。相同接种量(1×106),4周后处死小鼠,Sca-1+细胞组的瘤重及体积均大于Sca-1-细胞组(P<0.01)。结论Sca-1+细胞的成瘤性较Sca-1-细胞强。Objective To explored the tumorigenicity of sca-1 positive cells from Lewis lung carcinoma Cell Line in C57BL/6 Mice. Methods The sea-1 positive cells were sorted by magnetic activated cell sorting （MACS） from Lewis lung carcinoma Cell Line, the purity was analyzed by the method of fluorescence-actived cell sorting （FACS）. Unseparated, Sca-1^＋ and Sca-1^- populations were inoculated into the axillary fossa of C57BL/6 mice at the injection dose 1 × 10^6 , 5 × 10^5 , 1 × 10^,5 × 10^4 ,2 × 10^1 respectively. The mice were monitored for palpable tumor formation and were sacrificed after 4 weeks to assess tumor formation rate, volume and weight. Results FACS analysis shown the Sca-1 positive fraction was （8.06 ± 1.03） % in Lewis lung carcinoma Cell Linc. After isolation, the sca-1 positive fraction was raised to （93.92±1.07）%. Sca-1^＋ cells generated a tumor with 2 × 10^4cells compared with at least 5 × 10^5 needed for Sca-1^- cells, at the same injection dose （ 1 × 10^6cells） , the tumor generated by the Sca-1^＋ cells was larger than that of the Sca-1^- cells. Conclusion Sca-1^＋ cells have higher tumorigenicity than Sca-1^- cells.

关 键 词：LEWIS肺癌 干细胞抗原-1 肿瘤干细胞 致瘤性 

分 类 号：R734.2[医药卫生—肿瘤] R730.21[医药卫生—临床医学]