UC和其它肠道疾病肠道菌丛结构的ERIC-PCR指纹图谱分析  

The analysis of the DNA fingerprinting of intestinal flora in UC and other intestinal diseases

作　　者：张静[1] 韩英[2] 王继恒[2] 王志红[2] 

机构地区：[1]中国人民解放军总医院军医进修学院,北京100853 [2]北京军区总医院消化内科

出　　处：《胃肠病学和肝病学杂志》2007年第5期430-433,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的建立溃疡性结肠炎(UC)和其它肠道疾病的肠道细菌DNA指纹图谱以分析其肠道菌丛的整体差异。方法采集19例UC、11例急性胃肠炎、6例IBS患者及11例正常对照者的粪便标本,提取肠道细菌总DNA;应用ERIC-PCR技术建立各组肠道细菌DNA指纹图谱,分析UC、急性胃肠炎、IBS患者及正常对照者的肠道菌丛的整体差异。结果经分析发现四组研究对象的肠道菌丛存在整体差异:UC组ERIC-PCR指纹图谱电泳DNA条带较少,IBS组、急性胃肠炎组和正常对照组ERIC-PCR指纹图谱电泳DNA条带多;UC组中有18个样本主带分布非常一致,均出现在约0.7 kb处,急性胃肠炎组主带分布也非常一致,均出现在约1.1 kb和0.8 kb处;正常对照组和IBS组主带位置无统一趋势。结论与IBS、急性胃肠炎和正常对照组相比较,UC组肠道优势菌群种类少;UC组和急性胃肠炎组各有分布较一致的主带;UC可能存在较单一的肠道优势细菌,推测其发病机制可能与特定的肠道优势细菌感染有关。Objective DNA fingerprinting was carried out to analyze the difference of intestinal flora between UC and other intestinal diseases. Methods The fecal samples of 19 UC,11 acute gastroenteritis,6 IBS and 11 healthy controis were collected. DNA fingerprinting was setup by using of ER IC （ enter bacterial repetitive intergenic consensus ）- PCR technology,and the total difference of their intestinal flora was analyzed. Results The DNA fingerprintings of the four groups were identified,and a significant differences was noticed between them. More bands in IBS,acute gastroenteritis and healthy groups, but fewer in the UC cases. Strikingly,one single predminant band （ 0.7 kb） of DNA fingerprinting was noticed in 18 UC samples ; two predominant bands （ 1.1 kb and 0.8kb） were noticed in acute gastroenteritis samples; the healthy control and IBS group did not show predominant bands. Conclusions Compared with the variety of intestinal flora of IBS,acute gastroenteritis and healthy groups, UC patients have less predominant species of bacterial flora. Either UC or acute gastroenteritis holds different predominant flora. UC seems to have a single predominant intestinal bacterial flora which might associtated with the pathogenisis of UC.

关 键 词：溃疡性结肠炎 肠道菌丛 肠杆菌基因间重复共有序列 DNA指纹图谱 

分 类 号：R575.2[医药卫生—消化系统]