儿童慢性中度铅中毒幼兔动物模型的建立  

Establishment of Animal Model for Study of Children with Moderate Blood Lead Levels with Young Rabbits

作　　者：余高妍[1] 颜崇淮[1] 余晓刚 左勇[2] 邹向宇[1] 吴胜虎[1] 徐健[1] 张燕萍[1] 

机构地区：[1]上海交通大学医学院附属新华医院上海市儿科医学研究所上海市环境与儿童健康重点实验室,上海200092 [2]上海交通大学医学院附属细胞生物研究室,上海200025

出　　处：《实用儿科临床杂志》2007年第19期1484-1486,1513,共4页Journal of Applied Clinical Pediatrics

基　　金：上海市环境与儿童健康重点实验室资助(04DZ0590406DZ22024)

摘　　要：目的建立慢性中度铅中毒幼兔模型,为研究儿童慢性中度铅中毒治疗提供理想模型。方法16只离乳(45 d)雄性健康新西兰幼兔适应性喂养1周后,随机分为对照和染铅组,8只/组。染铅组饲喂含5 mg/(kg.d)醋酸铅饲料,持续6周,建立慢性中度铅中毒幼兔模型。原子吸收分光光谱仪(AAS)测其血铅(BLLs),电感耦合等离子体质谱仪(ICP-MS)测尿铅和组织脏器铅,光镜下观察其各组织脏器病理损伤。结果染铅组BLLs和尿铅水平在最初几周快速升高,随后维持稳定,且全程BLLs始终控制在450μg/L内;与对照组比较,染铅组幼兔体质量增加、睾丸湿质量系数和海马湿质量系数显著下降(Pa<0.01)。肝脏湿质量系数和各组织脏器铅水平显著升高(Pa<0.01)。光镜下发现海马(CA1区)、肝脏和睾丸组织发生病理改变。结论慢性中度铅中毒幼兔模型可作为研究儿童慢性中度铅中毒治疗的理想模型。Objective To establish the animal model for the study of children with moderate blood lead levels in young rabbits, for the study of the ideal therapy for moderate lead poisoning in children. Methods Sixteen 45 - day - old male New Zealand rabbits were randomly divided into control and lead - exposed group,8 in each group. Rabbits in the lead - exposed group were treated with 5 mg/（ kg ·d） lead acetate in their forage for 6 weeks to establish moderate lead poisoning animal model. The blood lead levels（ BLLs）were determined by atomic absorption spectrometer（ AAS）, and the urine lead levels and the lead concentrations of tissue and organ were determined by inductively coupled plasma - mass spectrometry（ ICP - MS）. Histopathology in tissue and organ was observed under the light microscope. Results The BLLs and the urine lead levels in lead -exposed group step up rapidly in primal weeks, then retained at a steady levels. The BLLs exhibited moderate level BLLs during the lead exposure period. Compared with control group, the body weight gain, testis and hippocampus wet coefficient of the lead - exposed group significantly decreased（ P〈 0.01 ） ,liver wet coefficient and the lead concentrations of tissue and organ significantly increased（ P, 〈 0.01 ）. The pathological changes was found in the hippocampus tissue,liver tissue and testicle tissue under the light microscope. Conclusion It is suggested that the model can be used for the study of the ideal therapy for moderate lead poisoning in children.

关 键 词：铅 动物模型 幼年期 兔 

分 类 号：R-332[医药卫生]