人与巴马香猪无细胞真皮移植效果的比较  

Comparison of the Results of Miniature Pig and Human Acellular Dermal Matrix(ADM) Grafting

作　　者：郑书全[1] 葛良鹏[2] 魏泓[2] 

机构地区：[1]西南大学生命科学学院,重庆400715 [2]第三军医大学基础部实验动物学教研室,重庆400038

出　　处：《中国比较医学杂志》2007年第10期568-571,I0002,共5页Chinese Journal of Comparative Medicine

基　　金：国家"十五"重点科技攻关项目(2004A717B-03)资助

摘　　要：目的比较巴马香猪与人无细胞真皮植入大鼠后的相容性、血管化和收缩率等移植效果,为猪无细胞真皮替代人无细胞真皮用于深度创伤治疗提供依据。方法以机械去表皮法制备巴马香猪与人无细胞真皮经辐照处理后移植于Wistar大鼠全厚皮肤缺损创面进行初步的淋巴细胞浸润、成纤维细胞进入生长、血管化、新生皮肤和创面收缩率比较。结果移植1个月后,移植两种无细胞真皮的的创面均完全愈合,组织学检测表明:巴马香猪无细胞真皮与人无细胞真皮相比炎症的发生时间较长,血管化速度和细胞迁入生长缓慢,而愈合后创面收缩率差异不显著。结论巴马香猪无细胞真皮的生物相容性和血管化等移植效果不及人无细胞真皮,主要影响因素可能在于二者蛋白质的差异。Objective To compare the results of BAMA miniature pig and human acellular dermal matrix （ADM） grafting, based on a Wistar rat full-thickness skin wound model. Methods ADMs were obtained from BAMA miniature pig and human skin removed by dermatotome, and irradiated for a dose of 30 kGy. Then, the two ADMs were used to cover the fullthickness skin wound in Wistar rats. The changes of wound surface, including biocompatibility, process of vascularization and shrinkage rate, were recorded until concrescence. Results The wound surface of BAMA miniature pig and human ADM grafting healed about 1 month later. Histological examination showed that inflammatory reaction on BAMA miniature pig ADM was more prominent than that on human ADM. Also, vascularization and fibroblasts immigration into BAMA miniature pig ADM was slower than those into human ADM. However, there was no significant difference between their shrinkage rates. Conclusion The results of this study indicated that biocompatibility and vascularization of BAMA miniature pig ADM is not as good as those of human ADM, mainly caused by the difference between their protein composition.

关 键 词：巴马香猪 真皮 无细胞 生物相容性 血管化 收缩率 

分 类 号：R-332[医药卫生]