日粮补镁影响大鼠机体抗氧化状态的实验研究  

An Experiment Study on Effect of Magnesium on the Antioxidant Status in Rats

作　　者：孙爱萍[1] 安星兰[1] 王志红[1] 付强[1] 赵爽[1] 刘源[1] 

机构地区：[1]军事医学科学院实验动物中心,北京100071

出　　处：《中国比较医学杂志》2007年第10期599-602,共4页Chinese Journal of Comparative Medicine

摘　　要：目的研究缺镁后补镁对大鼠机体抗氧化能力的影响。方法试验分两个饲养阶段：0～3周和4～6周。基础日粮含镁0.07%。第一阶段3组动物均饲喂基础低镁日粮;第二阶段,一组继续饲喂基础低镁日粮,第二组饲喂0.27%镁（补无机镁0.2%）日粮,第三组饲喂0.27%镁（补有机镁0.2%）日粮。试验末测血清镁水平,丙二醛（melon deldehade,MDA）含量和抗氧化酶活性。结果在试验第一阶段,血清镁显著降低（P〈0.05）,第二阶段添加0.2%镁提高了血清镁和脏器抗氧化酶活性（过氧化氢酶;超氧化物歧化酶;谷胱甘肽过氧化物酶）（P〈0.05）,降低了MDA水平（P〈0.05）。并且添加有机镁比添加无机镁抗氧化酶活性更高（P〈0.05）。结论镁缺乏动物机体抗氧化能力下降,补镁0.2%或0.4%均可提高大鼠机体抗氧化能力。Objective The purpose of the present study was to detect the effect of magnesium on the body＇s antioxidant system. Methods The feeding periods were divided into parts of 0 to 3 weeks and 4 to 6 weeks. During the first period （0 - 3 weeks）, all the rats in three groups were fed with basal diet which contained only 0.07% magnesium. During the second period （4 ～ 6 weeks）, one group still continued to be fed the basal diet, the other two were given high Mg （0.27% Mg） supplemented by MgSO4 or by proteinate magnesium diet. Results During the first period, the level of plasma magnesium was significantly decreased （ P 〈 0.05）. Addition of 0.2% Mg in diet significantly increased the level of plasma magnesium and the activity of some antioxidases（ P 〈 0.05）and significantly decreased MDA level （P 〈 0.05）. There was a significant difference between effects of organic magnesium and inorganic magnesium on the activity of antioxidase （P 〈 0.05 ）. Conclusion Magnesiumdeficient animals show an increased susceptibility to oxidative stress.

关 键 词：镁 脂质过氧化 谷胱甘肽 过氧化氢酶 超氧化物歧化酶 谷胱甘肽过氧化物酶 

分 类 号：R151.4[医药卫生—营养与食品卫生学]