优化胶粘贴法建立裸鼠胃癌原位种植模型  

Establishment of Orthotopic Implant Model of Human Gastric Cancer in Nude Mice Using Glue Paste Technique

作　　者：李莹杰[1] 贺岩[2] 王齐敏[2] 刘淼[2] 金晓明[2] 

机构地区：[1]哈尔滨医科大学附属二院病理科,哈尔滨150086 [2]哈尔滨医科大学病理教研室,哈尔滨150086

出　　处：《中国实验动物学报》2007年第5期369-371,I0003,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：黑龙江省卫生厅(编号:006-088);黑龙江省哈医大二院青年基金(编号:N2006-08)

摘　　要：目的通过对两种胶粘贴法建立的胃癌原位种植动物模型的比较研究,为探讨胃癌的发病机制和实验治疗提供理想的动物模型。方法用OB胶和FS生物蛋白胶法分别建立胃癌原位种植动物模型,观察和比较两种方法所建立的模型肿瘤生长状况、转移情况和形态学变化。结果FS生物蛋白胶组未出现肿瘤大片坏死,腹水形成率为85.7%,幽门梗阻发生率为57.1%;而OB胶组肿瘤大片坏死发生率为100%,腹水形成率为14.3%,未出现幽门梗阻。FS生物蛋白胶组有三例出现了肺和脑转移。结论FS生物蛋白胶法建立的裸鼠胃癌原位种植动物模型能更好的模拟人胃癌患者的临床过程,为研究人胃癌转移机制和实验治疗提供理想的动物模型。Objective To compare two types of orthotopic implant models of human gastric cancer in nude mice using glue paste technique and to provide an ideal animal model for research in the field of pathogenesis and experimental treatment of gastric cancer. Methods The orthotopic implant models of human gastric cancer in nude mice were established using OB and FS organic glue paste technique, respectively, the tumor growth characteristics, metastatic and morphological characteristics were analyzed and compared. Results In the model using OB glue no serious tumor necrosis was observed, the rate of ascite formation was 85.7 %, the rate of pyloric obstruction was 57.1%. In the model using FS organic glue the rate of extensive tumor necrosis was 100%, the rate of ascites formation was 14.3%, no pyloric obstruction was observed, metastases in the lung and brain were observed in three cases. Conclusions The orthotopic implant model of human gastric cancer in nude mice using FS organic glue paste technique, simulating the clinic process of patients, has been successfully established. It provides an ideal animal model for studies on mechanism of metastasis and treatment of human gastric cancer.

关 键 词：胃肿瘤 肿瘤细胞 原位移植 疾病模型 裸小鼠 

分 类 号：R735.2[医药卫生—肿瘤]