辣椒素提取及纯化的研究  

A Study on Extraction and Determination of Capsaicinoid

作　　者：舒丹[1] 郑人源[2] 叶小金[1] 罗琴[1] 张涛[2] 

机构地区：[1]成都医学院 [2]成都医学院生物化学教研室,成都610083

出　　处：《成都医学院学报》2007年第2期112-114,共3页Journal of Chengdu Medical College

基　　金：四川省教育厅资助科研项目(项目编号2006B112)

摘　　要：目的以干辣椒为原料,研究采用壳聚糖树脂吸附提取辣椒碱及其纯化的工艺。方法:用NaOH溶液进行粗提取,提取液处理后用壳聚糖树脂柱吸附,用乙醇洗脱得到辣椒素溶液,重结晶得到淡黄色针状辣椒素晶体。采用香兰素-亚硝酸钠比色法测定辣椒素含量。结果:从干辣椒中提取得到的辣椒素收率为0.38%,辣椒素晶体纯度为91.20%。结论:采用该方法提取辣椒碱,方法操作简便,成本低廉,产率和纯度较高,结果较理想。Objective To extract and determinate capsaicinoid from capsicum with chitosan resin. Method Capsaicinoid was separated by means of 1% NaOH extracting initially. After degreasing, the capsaicinoid in extraction was absorped by chitosan resin and then eluted by ethanol. A straw yellow crystal of capsaicinoid was isolated from recrystallization. The crystal capsaicinoid was deteminated by vanitlin- sodium nitrite colorimetry. Results Capsaicinoid was extracted and purified. The yield was 0.38% and the purity of crystal was 91.20%. Conclusion The method was not only simple in process, but also high in yield and purity at low cost. It could be used for industrial production,

关 键 词：辣椒素 提取 纯化 含量测定 

分 类 号：TQ461[化学工程—制药化工]