莲心碱对大鼠脑缺血/再灌注后NOS和C-Fos蛋白表达的影响  

Effect of Liensinine on NOS and C-Fos Protein Expression on Focal Cerebral Ischemia-Reperfusion Injury in Rats

作　　者：余万桂[1] 邓德明[1] 陈庭煊[2] 张恒文[1] 张燕祥[1] 张道明[1] 刘莲[1] 

机构地区：[1]长江大学医学院,湖北荆州434000 [2]长江大学附属第一医院荆州市第一人民医院病理科,湖北荆州434000

出　　处：《长江大学学报（自科版）（下旬）》2007年第3期220-222,共3页Journal of Yangtze University

基　　金：湖北省卫生厅科研基金资助项目(编号:JXzb74)

摘　　要：目的:观察莲心碱对大鼠脑缺血/再灌注后一氧化氮合酶(NOS)和C-Fos蛋白表达的影响并探讨其作用机制。方法:将24只SD雄性大鼠随机平均分为假手术组、缺血/再灌组和莲心碱组。采用大鼠大脑中动脉线栓(MCAO)法制备局灶性脑缺血/再灌注模型,应用免疫组化法分别检测各组大鼠脑组织NOS和C-Fos蛋白表达。结果:与假手术组比较,大鼠脑缺血/再灌注24h后NOS和C-Fos蛋白的表达明显增多(P<0.01),莲心碱能明显减少大鼠梗死灶范围,减少脑缺血/再灌注后NOS和C-Fos蛋白表达(P<0.05)。结论:莲心碱对大鼠脑缺血/再灌注具有保护作用,其机制可能与抑制NOS和C-Fos蛋白的表达有关。Objective: Observe the Effect of Liensinine on NOS and C-Fos Protein Expression on focal cerebral ischemia-reperfusion injury in rats and investigate the protective mechanism of Liensinine.Methods: The model of focal cerebral ischemia-reperfusion injury in rats was made by suture-occluded method.24 male Sprague-Dawley rats were randomly divided into three groups. sham operation group,injury group,Liensinine group.The expression of NOS and C-FOS protein were detected by immunohistochemical method. Results: The expression of NOS and C-FOS protein significantly increased in injury rats and Liensinine significantly inhibited the expression of NOS and C-FOS.Conclusion: The protective mechanism of Liensinine is probably related to the inhibition of NOS and C-FOS expression.

关 键 词：莲心碱 脑缺血/再灌注 NOS C—Fos蛋白 

分 类 号：R363[医药卫生—病理学] R743.3[医药卫生—基础医学]