氯离子通道ClC-5在大鼠牙胚发育过程中的表达  

Expression of ClC-5 in rat tooth germ development

作　　者：侯晋[1] 段小红[1] 司徒镇强[1] 

机构地区：[1]第四军医大学口腔医学院口腔生物学教研室,陕西西安710032

出　　处：《华西口腔医学杂志》2007年第5期444-446,453,共4页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30371540)

摘　　要：目的研究氯离子通道ClC-5在大鼠牙胚发育过程中的表达情况,探讨其在牙齿发育过程中的作用。方法从不同发育阶段的大鼠牙胚中提取总RNA和蛋白,应用RT-PCR和Western blot检测ClC-5在大鼠牙胚发育过程中mRNA和蛋白的表达情况。结果ClC-5在牙胚发育的过程中存在时空特异性表达,其mRNA和蛋白都主要在牙胚发育的钟状晚期表达。结论ClC-5在大鼠牙胚发育过程中的这种时空特异性表达,提示其可能在牙齿发育的过程中发挥一定的作用。Objective To determine the expression and possible function of CIC-5 during the rat tooth germ development. Methods Isolated total mRNA and protein from the rat tooth germs which were at different stages of development. RT-PCR and Western blot were used to investigate the mRNA and protein expression of the ClC-5 in the rat tooth germ. Results The mRNA and protein of ClC-5 expressed in the late bell stage, but undetected in the early or middle bell stage. Conclusion These results showed the spatial temporal distribution of expression of ClC-5 during the different stages of the rat tooth germ development and suggested that it might contribute to the rat tooth germ development.

关 键 词：氯离子通道 牙胚 发育 

分 类 号：R78[医药卫生—口腔医学]