纳米微囊-VEGF复合体对创伤组织中MMP-9及TIMP-1mRNA表达的影响  

The effects of complex of nanosphere-VEGF on expression of MMP-9 and TIMP-1mRNA in wounded tissue

作　　者：彭湃[1] 夏文森[1] 宋保强[1] 杨力[1] 潘勇[1] 张卫民[2] 

机构地区：[1]第四军医大学西京医院整形科,西安710032 [2]广州解放军第421医院

出　　处：《陕西医学杂志》2007年第4期422-425,共4页Shaanxi Medical Journal

摘　　要：目的：探讨纳米微囊-VEGF复合体对创伤组织中MMP-9及TIMP-1mRNA表达的影响.方法：利用纳米微囊-VEGF复合体作用于兔耳慢性创面,实验分为转基因组（CW＋VEGF）、慢性创面组（CW）、非缺血创面组（NW）,通过反转录PCR方法依次观察术后1d、3d、7d、10d、14d损伤组织中MMP-9及TIMP-1mRNA表达的变化情况.结果：①MMP-9 mRNA在CW组3～14d的表达水平高于NW组在相应时间点的表达水平,具有显著差异（P＜0.05）;CW＋VEGF组在10d及14d表达水平显著高于CW组,具有显著性差异（P＜0.05）.②TIMP-1 mRNA在NW组和CW＋VEGF组3～14d的表达水平均高于其在CW组相应时间点的表达水平,具有显著性差异（P＜0.05）.结论：MMP-9的高表达及TIMP-1的低表达是慢性创面难愈合的重要机制;外源性VEGF基因通过上调TIMP-1的表达促进了慢性创面的愈合.Objective： To study the effects of complex of nanosphere-VECF ,n expression of MMP-9 and TIMP-1mRNA, and investigate its mechanisms of improving wounded tissue repairing. Methods： We excerted complex of nanosphere-VECF on wounded rabit ears, samples were divided into chronic wound ＋ VEGF-transgenic group （CW ＋ VEGF）, chronic wound group （CW） and nonischemia wound group（NW）,using RT-PCR was used to examine expression of MMP-9 and TIMP-1mRNA on 1d, 3d, 7d, 10d, 14d post operation. Results： ①The expression of MMP-9 mRNA of rabbits in CW was greater than in NW at the same time from 3d to 14d post operation . with statistical significance（P〈0. 05）. ②The expression of TIMP-1 mRNA in NW and CW＋VEGF was both greater than in CW at the same time from 3d to 14d post operation, with statistical significance （P〈0.05）. Conclusion： High expression of MMP-9 and low expression of TIMP-1 might be important mechanisms of chronic raw surface healing; exogenous VEGF gene transfection might promote wound healing through upregulating the expression of TIMP-1.

关 键 词：创伤和损伤 血管生成因子 纳米技术 明胶酶B 金属蛋白酶1组织抑制剂 

分 类 号：R575.2[医药卫生—消化系统]