瘤内缓释治疗对B16黑色素瘤小鼠影响的实验研究  

Slow intra-tumor release of drugs on B16 melonoma in mice

作　　者：卜洁琼[1] 于保法 

机构地区：[1]山东大学医学院免疫研究所,济南250012 [2]济南保法肿瘤医院,济南250012

出　　处：《山东大学学报（医学版）》2007年第10期988-991,共4页Journal of Shandong University:Health Sciences

摘　　要：目的探讨肿瘤内缓释治疗对B16黑色素瘤的影响及其机制。方法将皮下接种B16肿瘤细胞后的成瘤小鼠随机分成4组进行治疗:①生理盐水(NS)对照组,瘤内注射NS;②阿糖胞苷(Ara-C)对照组,瘤内注射Ara-C;③单纯缓释治疗组,瘤内注射Ara-C+缓释液;④免疫缓释液治疗组,瘤内注射Ara-C+缓释液+二硝基苯(DNP)。4 d后重复治疗,观察各组小鼠肿瘤的生长情况。两次治疗后1周分离瘤体,HE及网状纤维、弹力纤维、胶原纤维染色,观察瘤体病理改变,用流式细胞术检测脾内CD4+T细胞、CD8+T细胞浸润情况。结果缓释治疗能够控制肿瘤的生长;HE染色显示,两缓释治疗组瘤内出现大片坏死区域,纤维特殊染色显示,两缓释治疗组三种纤维含量均明显增加(P<0.01);流式细胞术检测表明,缓释治疗组的CD4+T细胞数量明显增多(P<0.05),但CD4+/CD8+T比值无明显变化。结论瘤内缓释治疗通过直接破坏肿瘤细胞及增加肿瘤抗原性,从而发挥明显的抗肿瘤作用,为肿瘤治疗提供了新的思路。Objective To explore the effects and mechanism of slow intra-tumor release of drugs on B16 melonoma in mice. Methods Tumor-bearing mice were randomly divided into four groups： the normal saline group, the Ara-C group, the Ara-C plus releasing drugs group and the Ara-C plus releasing drugs and DNP group. Four days later, mice were treated again and the gross tumor volume was determined. Then 1 week later, the contents of three fibers were detected in the interstitial substances of the tumor and the infiltrations of T cells were determined in the splenic organs. Results HE staining results showed that there was a large part of stenosis in the tumor, the fibers in the matrix of tumor were significantly increased and the CD4^＋ T cells were also significantly increased in the two slow-release groups. Conclusion Slow-release of drugs into the tumor is one of the effective managements for directly inhibiting tumor growth and preventing micro-metastasis.

关 键 词：黑色素瘤 缓释治疗 肿瘤内化疗 二硝基苯酚 抗原修饰 

分 类 号：R737.14[医药卫生—肿瘤]