成人喉乳头状瘤组织中HPV/E7、ki-67表达与微血管密度的相关性  

Relationship between the expression of HPV/E7,ki-67 and The microvessel dentisy in laryngeal papilloma in adults

作　　者：赵秋良[1] 李海燕[1] 

机构地区：[1]山东大学医学院,济南250012

出　　处：《山东大学学报（医学版）》2007年第10期1030-1033,共4页Journal of Shandong University:Health Sciences

摘　　要：目的探讨人乳头状瘤病毒、细胞增殖活性及新生血管的生成在喉乳头状瘤中的作用。方法选取21例成人喉乳头状瘤组织标本,以14例声带息肉和14喉鳞癌组织标本为对照组,PCR方法检测HPV16/E7的表达,免疫组化法检测Ki-67、CD34的表达。结果HPV16/E7在声带息肉、成人喉乳头状瘤和喉鳞癌组织中的阳性表达率分别为:14.3%、66.7%、85.7%;Ki-67标记指数(labeling index,LI)分别为6.30±2.03、13.73±3.42、25.55±3.92;MVD分别为3.97±1.38、14.61±1.64、25.34±2.63;差异有统计学意义(P<0.01)。HPV16/E7,CD34和Ki-67呈正相关(r=0.854,P<0.01;r=0.878,P<0.01)。结论Ki-67、MVD与细胞的增殖活化程度明显相关,可以作为反映成人喉乳头状瘤恶变潜能的指标之一。Objective To explore the role of HPV16/E7, cellular proliferation activity and tumor angiogenesis in laryngeal papilloma（LP） in adults. Methods Twenty tissues of LP in adults were selected in the experimental group, and 14 tissues of vocal cord polyps （VCP） and 14 tissues of laryngeal squamous cell carcinoma（LSCC） were enrolled in the control groups. Expression of HPV16/E7 was determined by the polymerase chain reaction（PCR） method, and of Ki- 67 and CD34 by the immuneohistochemical SP method. Results The expression rate of HPV16/E7 in VCP, LP and LSCC was 14.3%, 66.7%, and 85.7%, respectively. The labeling index（H） of Ki-67 was 6.30±2.03, 13.73 ± 3.42, and 25.55 ± 3.92, respectively. The number of MVD was 3.97 ± 1.38,14.61 ± 1.64, and 25.34 ± 2.63, respectively. The difference was significant （ P 〈 0.01 ）. The level of HPV16/E7, CD34 was positively correlated with that of Ki-67 in the tissues（r =0.854, P 〈0.01; r =0.878,P 〈0.01）. Conclusion Ki-67 and MVD highly correlates with tumor angiogenesis, cellular proliferation activity and pathological types, and they can serve as malignant indicators for LP in adults.

关 键 词：喉肿瘤 喉乳头状瘤 增殖细胞抗原 CD34 

分 类 号：R739.65[医药卫生—肿瘤]