马铃薯茎段愈伤组织培养体系的优化  

Optimizing for callus tissue culture system for the stem of Solanum tuberosum

作　　者：罗源[1] 陈耀锋[1] 李春莲[1] 余玲[1] 任慧莉[1] 李映梅[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨陵712100

出　　处：《西北农林科技大学学报（自然科学版）》2007年第10期159-162,共4页Journal of Northwest A&F University(Natural Science Edition)

摘　　要：以克新12号、克新13号、东农303和早大白4种马铃薯试管苗茎段为材料,研究各品种的组织培养体系。结果发现,克新13号和克新12号的最适愈伤组织诱导培养基为MS+30 g/L蔗糖+8 g/L琼脂+2 mg/L 6-BA+1 mg/L 2,4-D;东农303为MS+30 g/L蔗糖+8 g/L琼脂+2 mg/L 6-BA+0.5 mg/L 2,4-D;早大白为MS+30 g/L蔗糖+8 g/L琼脂+2 mg/L 6-BA+0.5 mg/L 2,4-D。用上述最适诱导培养基培养,各品种的愈伤诱导率分别为100%,100%,100%和90%。克新13号愈伤组织分化的最佳培养基为MS+30 g/L蔗糖+8 g/L琼脂+4.0mg/L 6-BA+0.1 mg/L NAA+1.0 mg/L GA3;克新12号和早大白为MS+30 g/L蔗糖+8 g/L琼脂+2.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L GA3;东农303为MS+30 g/L蔗糖+8 g/L琼脂+2.0 mg/L 6-BA+0.1 mg/LNAA+1.5 mg/L GA3。用上述最适诱导培养基培养,各品种的分化率分别为80%,90%,100%,76.7%。Four potato cultivars, Kexin No. 12, Kexin No. 13,Dongnong303 and Zaodabai were cultivated in vitro from stem explants. The results showed that the optimal medium of callus for Kexin No. 12 and Kexin No. 13 was MS＋30 g/L sucrose＋8g/L agar ＋2 mg/L6-BA＋ 1 mg/L 2,4-D;Dongnong 303 was MS ＋2 mg/L 6-BA q-0.5 mg/L 2,4-D;the optimal medium of Zaodabai was MSq-30 g/L sucrose＋8 g/L agar ＋2 mg/L 6-BAq-0. 5 mg/L 2,4-D; the adventitious callus induction rate were 100%, 100%, 100% and 90%, respectively by using these optimal induced medium. The best medium for adventitious bud differentiation from stem callus was MS-k30 g/L sucrose-k8 g/L agar＋4. 0 mg/L 6-BA＋0.1 mg/L NAA＋l. 0 mg/L GA3 for Kexin13;MS＋30 g/L sucrose＋8 g/L agar＋2.0 mg/L 6-BA＋0.5 mg/L NAA＋1. 0 mg/L GA3 for Kexin12;MS＋30 g/L sucrose＋8 g/L agar＋2.0 mg/L 6-BA＋0.1 mg/L NAA＋1. 5 mg/L GA3 for Dongnong 303;MSq-30 g/L sucrose＋8 g/L agar＋2. 0 mg/L 6-BAq-0.5 mg/L NAA＋1. 0 mg/L GA3 for Zaodabai. Under these optimal induced medium, the rates of adventitious bud differentiation were 80%, 90%, 100% ,and 76.7%, respectively.

关 键 词：马铃薯 克新12号 克新13号 东农303 早大白 愈伤诱导 愈伤分化 

分 类 号：S532.035.3[农业科学—作物学]