荧光原位杂交技术预测人大肠癌细胞内在放射敏感性的实验研究  

The experiment study on predicting the radiosensitivity of human colorectal cancer cell by fluorescence in situ hybridization

作　　者：邢春根[1] 李国强[1] 周丽英[1] 蒋银芬[1] 刘玉龙[1] 薛永权[2] 陆雪官[1] 吴亚芳[2] 戴宏[1] 田野[1] 

机构地区：[1]苏州大学附属第二医院,苏州215004 [2]苏州大学附属第一医院,苏州215006

出　　处：《辐射研究与辐射工艺学报》2007年第5期308-311,共4页Journal of Radiation Research and Radiation Processing

基　　金：国家卫生部科研基金(WKJ2006-2-027)资助

摘　　要：探讨荧光原位杂交技术预测人大肠癌细胞内在放射敏感性的可行性。应用荧光原位杂交技术检测人大肠癌动物模型照射后癌细胞2号染色体易位畸变并分析其与照射后肿瘤生长曲线的相关性。结果表明,放疗使Lovo移植瘤的增长明显延缓,Lovo细胞的放射敏感性高于SW480细胞。X射线照射后肿瘤荧光原位杂交技术检测结果显示:Lovo细胞的2号染色体诱导易位畸变量显著大于SW480细胞(p<0.05)。荧光原位杂交技术能快捷、可靠地预测人大肠癌细胞的放射敏感性,从而为临床大肠癌病人放疗的选择提供重要参考依据。To study the correlation between the number of radiation-induced translocations of chromosome 2 and the cell survival fraction after irradiation using fluorescence in situ hybridization, and discuss the feasibility to predict the radiosensitivity of human colorectal cancer cell using FISH. The result shows that The growth of implanted tumor had been restrained after irradiation. The sensitivity of Lovo cell was higher than SW480. The result of FISH detection to implanted tumor resected indicated that the number of chromosome 2 aberration of Lovo cell was higher than SW480 significantly （p〈0.05）. Fluorescence in Situ Hybridization can be used to predict the intrinsic radiosensitivity of human colorectal cancer cell conveniently and reliably. It provides important references for the selection of radiotherapy in the patients suffering colorectal cancer clinically.

关 键 词：荧光原位杂交 预测 大肠癌细胞 放射敏感性 

分 类 号：R735.34[医药卫生—肿瘤] R73-36[医药卫生—临床医学]