CEA-rV负荷树突状细胞与CIK细胞共培养后对裸鼠结肠癌移植瘤生长的抑制作用  

Inhibitive Effect of CIK Cells Cocultured With Dendritic Cells Loaded With CEA Recombinant Virus on Human Colonic Carcinoma in Nude Mice

作　　者：田慧[1] 祁岩超[1] 王远东[2] 杨波[1] 卢敏莹[1] 申鸿卓[1] 潘东晓[1] 

机构地区：[1]广州医学院附属肿瘤医院生物工程室,广东广州510094 [2]广州医学院附属肿瘤医院胸外科

出　　处：《实用预防医学》2007年第5期1380-1383,共4页Practical Preventive Medicine

基　　金：广东省科技厅科研基金资助(编号:2006B35502013)

摘　　要：目的探讨负荷了CEA-rV的树突状细胞与CIK细胞共培养后在裸鼠体内特异性的抑瘤作用。方法在Balb/c裸鼠颈背皮下接种Lovo结肠癌细胞,次日起连续6d给予不同的效应细胞(CIK、DC+CIK、CEA-rV+DC+CIK)治疗,观察其对结肠癌生长的抑制作用。结果裸鼠体内实验表明,CEA-rV+DC+CIK能够显著抑制CEA阳性的Lovo结肠癌细胞的生长,其抑瘤率可达51.2%,明高于CIK组的25.1%以及DC+CIK组的28.5%。结论负荷CEA-rV的DC与CIK细胞共培养后在裸鼠体内对CEA阳性的Lovo结肠癌有特异性抑瘤作用。Objective To study the in vivo CEA - specific anti - tumor immune response induced by ClK cells cocultured with dendritic cells loaded with CEA- recombinant vaccinia virus. Methods Balb/c nude mice were subcutaneously transplanted with Lovo colonic carcinoma cells at the exponential growth period and then treated with different effector cells （CIK, DO ＋ CIK, and CEA - rV ＋ DO ＋ CIK） for consecutive 6 days from the 2nd day after Lovo transplantation Results The inhibitive ratio was significantly higher in CEA- rV＋ DC＋ CIK group （51.2%） than in CIK group （25.1% ） or DC＋ CIK group （28.5 % ）. Conclusions The CIK ceils cocultured with dendritic ceils loaded with CEA recombinant virus show a special killinq activity to CEA positive Lovo colonic carcinoma cells in nude mice in vivo,

关 键 词：树突状细胞 CIK细胞 CEA—rV Lovo结肠癌细胞 

分 类 号：R-332[医药卫生]