体外导入外源IL-10基因对肝星状细胞Ⅰ、Ⅲ型胶原分泌的影响  

Effects of IL-10 transgene into HSCs on the secretary of type Ⅰcollagen and type Ⅲ collagen

作　　者：胡良凯[1] 孙巧玲[2] 梁立维[1] 张建民[1] 李定国[2] 

机构地区：[1]上海市杨浦区市东医院消化内科,上海市200438 [2]上海交通大学附属新华医院消化内科

出　　处：《实用诊断与治疗杂志》2007年第11期809-811,881,共4页Journal of Practical Diagnosis and Therapy

基　　金：上海市杨浦区青年课题基金资助(项目编号:2005AA002)

摘　　要：目的：利用重组复制缺陷型腺病毒AdIL-10将外源IL-10基因导入肝星状细胞并观察其对肝星状细胞Ⅰ、Ⅲ胶原分泌的影响。方法：用传至1～2代的大鼠肝星状细胞以2×10^5/孔接种于24孔板,按每组6复孔分为三组：（1）单纯肝星状细胞培养组;（2）AdGFP对照组：肝星状细胞培养24h后取病毒AdGFP以感染复数50感染肝星状细胞;（3）AdIL-10组：肝星状细胞培养24h后取病毒AdIL-10以感染复数50感染肝星状细胞。3d后分别收集各组肝星状细胞,提取总RNA。结果：与单纯肝星状细胞培养组相比,AdIL-10组的IL-10mRNA表达明显增强（P〈0.01）,而AdGFP对照组的IL-10mRNA表达差异无统计学意义（P〉0.05）;AdIL-10组的Ⅰ型胶原和Ⅲ型胶原mRNA表达明显降低（P〈0.01）,而AdFP对照组的Ⅰ型胶原和Ⅲ型胶原mRNA表达差异无统计学意义（P〉0.05）。结论：重组腺病毒AdIL-10感染肝星状细胞后可抑制肝星状细胞Ⅰ、Ⅲ胶原分泌。　Objective To investigate effects of IL-10 transgene into HSCs on the secretary of type Ⅰcollagen and type Ⅲ collagen using recombinant replication deficient adenoviruses carrying rat IL-10 cDNA（AdIL-10）.Methods Primary cultured HSCs were seeded on 24-well plates at a density of 2×10^5 cells per well.HSCs were subjected to different treatment described as control group,AdGFP group（HSCs infected with AdGFP at a dose of MOI 50）and AdIL-10 group（HSCs infected with AdIL-10 by MOI 50）.At the end of culture,total RNA was isolated from HSCs.Results IL-10 mRNA expression of AdIL-10 group was much higher than control group（P〈0.01）,while such expression remained the same in AdGFP group and control group（P〉0.05）.The changes of type Ⅰ and type Ⅲ collagen expressions of AdIL-10 group were much lower than control group（P〈0.01）,while such expression remained the same in AdGFP group and control group（P〉0.05）.Conclusion AdIL-10 could infect HSCs and decrease type Ⅰcollagen and type Ⅲ collagen mRNA expression.

关 键 词：白介素-10 重组腺病毒 肝星状细胞 

分 类 号：R575.2[医药卫生—消化系统]