清热解毒法对脑出血大鼠的神经保护作用  

Neurological protective effect of heat-clearing and detoxication treatment for intracerebral hemorrhage in rats

作　　者：周庆博[1] 李鲁扬[2] 贾青[3] 毕建忠[1] 邵念方[4] 

机构地区：[1]山东大学第二医院神经内科,山东济南250033 [2]山东大学齐鲁医院保健科,山东济南250012 [3]山东医学科学院基础医学研究所,山东济南250012 [4]山东中医药大学附属医院,山东济南250012

出　　处：《山东大学学报（医学版）》2007年第9期947-950,954,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金项目(Y2002C26)

摘　　要：目的:研究遵清热解毒法制成的脑宁康颗粒对脑出血大鼠脑组织的神经保护作用机制。方法:实验大鼠随机分为5组:假手术组、脑出血模型组、清热解毒组(1.2、2.4和4.8 mg.kg-1),采用胶原酶Ⅶ诱导大鼠脑出血模型,术后2 h,清热解毒组给予脑宁康颗粒灌胃,另2组给予等容量生理盐水,连续用药(1次/日),分别在给药3 d和7 d后取脑组织,应用HE染色观察大鼠脑组织病理形态学改变,应用免疫组织化学法检测脑出血周围脑组织凝血酶受体(PAR-1)的表达,干湿比重法检测脑组织水肿。结果:脑宁康颗粒能够明显改善脑出血大鼠脑组织病理形态学改变,抑制脑组织PAR-1表达,减轻脑组织水肿。结论:脑出血后脑组织PAR-1表达增强,PAR-1高表达可能介导了神经细胞损伤和脑水肿,清热解毒法对脑出血大鼠的神经保护作用,可能与其抑制脑出血后脑组织PAR-1表达和减轻脑水肿相关。Objective： To investigate the mechanism of neurological protective effect of the Naoningkang granula on brain tissues of rats with intracerebral hemorrhage （ICH）. Methods： Rats were randomly divided into five groups： the sham operation group, the ICH model group and the experimental groups （ 1.2, 2.4 and 4.8 mg· kg^-1 Naoningkang, respectively）. Collagenase Ⅶ was injected nto the caudate nucleus to establish a rat model of ICH. After 2 hours, the Naoningkang granula was given to the experimental groups by gastrogavage, and saline was given to the other 2 groups by gastrogavage, At 3 and 7 days after treatments, brain tissues were taken. HE staining was used to observe the pathological changes. An immunohistochemical method was used to determine the PAR-1 content in brain tissues surrounding hematoma and the dry-wet weight method to assay the water content of brains. Result： The Naoningkang granula significantly improved the pathological changes, inhibited PAR-1 expression and reduced brain edema, Conclusion： PAR-1 expression is up-regulated after ICH in rats, which might play an important role in inducing nerve cell damage and brain edema. The heat-clearing and detoxication method has a significant neurological protective effect on ICH rats probably by inhibiting PAR-1 expression and reducing brain edema.

关 键 词：脑出血 大鼠 清热解毒 凝血酶受体 脑水肿 

分 类 号：R743.34[医药卫生—神经病学与精神病学]