食管鳞癌多阶段癌变中14-3-3σ和P53表达特征及相关性分析  

Characterization and correlation of 14-3-3σ and P53 protein expressions in multi-stage carcinogenesis of esophageal squamous cell carcinoma

作　　者：齐义军[1] 马远方[1] 杜耀武[1] 刘广超[1] 张蕾蕾[2] 张国民[2] 施巩宁[3] 张双林[3] 李勇[3] 何占锋[3] 何庆瑜[4] 

机构地区：[1]河南大学免疫学研究所 [2]河南大学淮河医院病理科,河南开封475000 [3]河南大学淮河医院胸心外科,河南开封475000 [4]暨南大学生命与健康工程研究院,广东广州510632

出　　处：《第四军医大学学报》2007年第20期1834-1837,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30700366)

摘　　要：目的:探讨14-3-3σ和P53蛋白表达在食管鳞癌和癌旁正常黏膜上皮、各级癌前病变中的变化特征及相关性.方法:采用免疫组织化学方法检测14-3-3σ和P53蛋白在60例不同分化程度食管鳞癌和癌旁正常食管黏膜上皮、各级癌前病变中的表达变化.结果:癌旁正常食管黏膜上皮中均可检测到不同程度的14-3-3σ蛋白表达(表达量8.22),随着癌前病变进展,14-3-3σ的表达率和表达量逐渐下降;低分化鳞癌中14-3-3σ的表达量最低(表达量0.45),完全失表达率为64%(7/11).P53蛋白在鳞癌中表达率最高67%(27/40),表达量为4.86,而只有25%(6/24)癌旁正常上皮表达P53蛋白(表达量0.42).14-3-3σ和P53蛋白的相关性分析表明二者呈显著负相关(P<0.05).结论:P53蛋白可能通过负调控14-3-3σ基因的表达参与食管癌变多阶段演进过程,14-3-3σ和P53可能成为食管癌早期诊断及高危人群筛查的分子指标,同时也可能成为食管癌生物预防和疗效评价的新靶点.AIM： To characterize the 14-3-3σ and P53 protein expressions during multi-stage carcinogenesis of esophageal squamous cell carcinoma （ESCC） and in normal esophageal mucous epithelium. METHODS ： Immunohistoehemistry （ ABC ） methods were used to determine 14-3-3σ and P53 protein expressions in 60 cases of ESCC, nearby matched normal esophageal epithelium and a variety of ESCC precursors, RESULTS ： High expression of 14-3-3σ was found ubiquitously in normal esophageal epithelium with a digitalized average value of 8, 22 in expression. Protein 14-3-3σ was down-regulated stepwise during the development of esophageal carcinoma from normal epithelium, Sixty-four percent of poorlydifferentiated squamous cancer lost 14-3-3σ expression with the digitalized value of 0, 45, However, pattern of P53 protein expression was in contrast to 14-3-3σ, Sixty-seven percent of esophageal cancer expressed P53 at a high level with a quantitated amount of 4.8, In nearby normal epithelium of esophagus, the P53 protein expression was low with a digitalized value of 0, 42. Significant negative correlation between 14-3-3σ and p53 expressions was revealed by Spearman rank correlation analysis （ P 〈 0. 05 ）. CONCLUSION： The aberrant expression pattern of 14-3-3σ and p53 may contribute to the formation and progression of ESCC and may predict the prognosis of ESCC patients. Proteins 14-3-3σ and P53 have the potential to become biomarkers for early diagnosis of esophageal cancer and end-point molecules of ESCC prevention.

关 键 词：食管肿瘤 癌 鳞状细胞 14-3-3σ 基因 P53 分子标记物 

分 类 号：R730.231[医药卫生—肿瘤]