银腺杨转Cry I Ac和API双价抗虫基因的研究  

Transformation of Cry I Ac and API Two Insect-resistant Genes to Poplar(Populus alba×P.glandulosa)

作　　者：李科友[1] 樊军锋[1] 赵忠[1] 李玲[2] 朱海兰[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100 [2]中国林业科学研究院林业研究所,北京100091

出　　处：《林业科学研究》2007年第5期699-704,共6页Forest Research

基　　金：国家转基因植物研究与产业化专项"耐盐抗虫转基因杨树新品种选育(JY03-B-26-02)"

摘　　要：在建立以银腺杨（84K）叶片为外植体的再生系统基础上，通过农杆菌介导法把CryⅠ4c和API双价抗虫基因导人银腺杨（84K）基因组中。转化杨树叶片，在含有卡那霉素的培养基上诱导不定芽和诱导生根，获得了400株卡那霉素抗性转化再生植株。抗性植株经PCR检测，有70株呈阳性。通过Southern杂交和ELISA检测进一步证明CryⅠ4c和API双价抗虫基因已整合到银腺杨（84K）基因组中，拷贝数1～2个，并得到了表达。转化植株用杨扇舟蛾幼虫进行饲虫试验，结果表明昆虫幼虫的死亡率高达60．0％～80．0％。同时，存活幼虫的生长发育也受到了明显抑制。本研究结果为杨树抗虫育种提供了新的种质资源。Based on the established leaf explant regeneration system of poplar （Populus alba × P. glandulosa cv. 84K）, Cry I Ac and API fused genes were introduced into the genome of poplar 84K by Agrobacterium tumefaciens- mediated transformation. Through successive selection in the period of shoot and root induction under high level ka- namycin-pressure, 400 kanamycin-resistant regenerated plants were obtained, 70 of them were positive resulted by PCR detection. Southern hybridization and ELISA detection were further showed that Cry I Ac and API gene had been integrated into the genome of 84K with one or two copies and expressed. The transformation plants were given insect feeding test with larvae of Clostera anachoreta. The results showed the mortality of insect larvae was accounted for 60.0% - 80.0%, and the growth of the survival larvae were seriously inhibited. These plants could provide new resources for poplar insect-resistant breeding.

关 键 词：银腺杨(84K) CryⅠ 4c和API基因 转化 抗虫性 

分 类 号：S722.3[农业科学—林木遗传育种]