两种MAP激酶共同调节蚕豆气孔保卫细胞中ROS信号途径研究  

Study on Two MAP Kinases Co-directe ROS Signaling in Vicia Guard Cell

作　　者：江静[1] 雷勇[1] 

机构地区：[1]河南大学植物逆境生物学重点实验室,河南开封475001

出　　处：《河南大学学报（自然科学版）》2007年第6期617-621,共5页Journal of Henan University:Natural Science

基　　金：国家自然科学基金项目(30470895);河南省高校新世纪优秀人才支持计划项目(2005HANCET-06)

摘　　要：运用表皮条生物学分析、激光共聚焦扫描及膜片钳技术,在MEK1/2和p38MAP激酶专一性抑制剂PD98059和SB203580处理下,观察促分裂原活化蛋白激酶(mitogen-activated protein kinase,MAP激酶)家族成员介导蚕豆保卫细胞中氧化信号机制.结果表明,PD98059能阻断并逆转ABA和H2O2抑制质膜内向K+电流、ABA诱导H2O2产生及气孔关闭,SB203580也表现相似的作用.因此,两种MAP激酶可能共同调节ABA诱导ROS产生和气孔关闭.In this work, MEK1/2 and p38 MAP kinase-like may play important roles in H2O2 signaling in Vicia guard cells using epidermal strip bioassay, laser-scanning confocal microscopy and whole-cell patch clamp analysis. Either PD98059, a specific inhibitor of MEK1/2, or SB203580, a specific inhibitor of p38MAP kinase, blocked H2O2-or ABA-induced stomatal closure, ABA-induced H2O2 generation, and the decrease of inward K^＋ currents in the plasma membranes of guard cells caused by application of ABA and H2O2. These results suggest that MEK1/2 and p38 MAP kinase-like are synchronously required for ABA-induced ROS signaling in guard cells.

关 键 词：MEK1/2 P38 MAP KINASE 蚕豆 保卫细胞 ROS信号 

分 类 号：Q257[生物学—细胞生物学]