paxillin在大肠癌中的表达及其生物学意义  

Expression of paxillin in colorectal carcinoma and its biological significance

作　　者：杨红军[1] 丁彦青[1] 周军[1] 杨磊[1] 

机构地区：[1]南方医科大学病理学教研室,广东广州510515

出　　处：《实用肿瘤杂志》2007年第5期412-415,共4页Journal of Practical Oncology

基　　金：国家自然科学基金资助项目(30170423)

摘　　要：目的检测桩蛋白(paxillin)在大肠癌组织中的表达,并探讨其在大肠癌发生、发展中的意义。方法采用Western-blot及免疫荧光结合激光共聚焦显微镜对大肠正常黏膜、原发大肠癌及淋巴结转移癌组织的paxillin表达水平进行检测。结果大肠黏膜由良性向恶性转变及转移过程中,paxillin表达水平逐渐升高;有淋巴结转移的大肠癌组织paxillin表达水平明显高于无淋巴结转移者;paxillin表达水平与癌组织浸润肠壁深度呈正相关,而与癌细胞分化程度无明显相关性。结论paxillin与大肠癌的浸润转移有关,可作为大肠癌发生、发展的预测指标之一。Objective To detect the expression of paxillin in colorectal carcinoma（CRC） and to investigate the significance of paxillin in tumorigenesis and progression of CRC.Methods The expression of paxillin in normal colorectal mucosae,primary CRCs and lymph node metastases was detected using Western-blot and immunofluorescence in combination with confocal laser scanning microscopy.Results The level of paxillin expression increased gradually during the transition from normal colorectal mucosae to malignant lesions and metastasis.The expression of paxillin in CRCs with lymph node metastasis was significantly higher than that in CRCs without lymph node metastasis.The paxillin expression,showing no correlation with the differentiation of CRC,was positively correlated with the infiltrating depth of carcinomas.Conclusions Paxillin is related to the infiltrating and metastasis of CRC.The level of paxillin expression might be a valuable marker for the carcinogenesis and progression of the CRC.

关 键 词：结肠直肠肿瘤 桩蛋白 荧光免疫测定 激光共聚焦显微镜 肿瘤转移 

分 类 号：R735.34[医药卫生—肿瘤]