赤芍总苷对大鼠缺血损伤心肌细胞凋亡的保护作用  

The protective effect of total paeony glycoside on apoptosis in myocardial ischemic rats

作　　者：沈贤[1] 莫晓燕[1] 杜晓阳[2] 

机构地区：[1]西安交通大学生命科学与技术学院,陕西西安710049 [2]西安交通大学医学院,陕西西安710061

出　　处：《中国药理学通报》2007年第10期1300-1305,共6页Chinese Pharmacological Bulletin

基　　金：西安市工业科技计划资助项目(NoGG04066);陕西省中医管理局基金资助项目(No2005007)

摘　　要：目的研究赤芍总苷(TPG)对异丙基肾上腺素(ISO)诱导的心肌缺血损伤大鼠细胞凋亡的保护作用及其机制。方法Rona法复制大鼠心肌缺血损伤模型,检测和对比分析正常对照组、ISO损伤组、TPG防治组、TPG治疗组和心得安阳性对照组的心肌细胞早期凋亡率、心肌组织凋亡相关基因Bax、Bcl-2及其蛋白的相对表达量。结果与ISO损伤组比较,TPG防治组和治疗组各项指标均有不同程度的改善,表现为大鼠早期心肌细胞凋亡率和促凋亡基因Bax及其蛋白的表达量明显降低,而抑凋亡基因Bcl-2及其蛋白的表达量有所增高,Bcl-2/Bax蛋白比值明显高于ISO损伤组。结论TPG对ISO诱导的大鼠心肌缺血损伤时的细胞凋亡具有保护作用,药用机制可能与激活Bcl-2基因及其蛋白表达,抑制Bax基因及其蛋白表达,提高Bcl-2/Bax蛋白比值,从而有效抑制心肌细胞凋亡有关。Aim To further investigate the protective effect and mechanisms of total paeony glycoside （TPG） on apoptosis in experimental myocardial ischemic rats. Methods The myocardial ischemic model of rats was established by subcutaneous injection of isoprenaline （ISO）, and early apoptosis rate of myocardial cells and the expression of apoptosis related genes Bax, Bcl- 2 and proteins in myocardial tissues were analyzed and contrasted among five groups, including normal control, ISO injury, TPG prevention and treatment, TPG treatment and positive control. Results Compared with ISO injury group, all of indexes had improved in various degrees in TPG prevention and treatment group and TPG treatment group, such as the decrease of early apoptosis rate,expression of Bax gene and protein, the increase of Bcl-2 gene and protein and the ratio of Bcl- 2 protein/Bax protein. Conclusion TPG has protective effect on apoptosis in ischemic myocardium induced by ISO. The pharmacological mechanism may relate to the activation of the Bcl-2 gene and protein expression, the inhibition of Bax gene and protein expression, as well as the increase of Bcl-2/Bax ratio.

关 键 词：赤芍总苷 异丙基肾上腺素 心肌缺血 细胞凋亡 

分 类 号：R-332[医药卫生] R282.71