复智散对阿尔采末病模型小鼠治疗作用的研究  

Effect of Fuzhisan on Alzheimers disease model mice

作　　者：李绪领[1] 王德生[1] 孙曼霁[2] 赵宝全[2] 汤颖[1] 张婷[2] 曲恒燕[2] 李前[2] 

机构地区：[1]哈尔滨医科大学第一临床医学院神经内科,黑龙江哈尔滨150001 [2]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国药理学通报》2007年第10期1322-1325,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30670726);黑龙江省自然科学基金重大项目资助课题(NoZJY03-5)

摘　　要：目的观察复智散（FZS）对Aβ25-35诱导的阿尔采末病（AD）模型小鼠的治疗作用。方法Aβ25-35侧脑室注射制作AD小鼠模型，以不同剂量的FZS灌胃治疗，通过Morris水迷宫和小鼠穿梭程序自动控制仪检测小鼠的认知功能，采用比色法测定小鼠脑内胆碱乙酰基转移酶（ChAT）、乙酰胆碱酯酶（AChE）活性及乙酰胆碱（ACh）含量。结果不同剂量的FZS不仅对AD小鼠的认知功能有明显的改善作用，且能增加AD小鼠脑内的ChAT活性及ACh含量，并降低AChE活性。结论FZS在1．25～10g·kg^-1剂量范围内均有治疗小鼠AD的作用，但用1．25g·kg^-1剂量时治疗效果最佳。Aim To examine the effects of Fuzhisan （FZS） on the Alzheimer＇s disease （AD） model mice induced by intracerebroventricular Aβ25-35. Methods The AD model of mice was established by the injection of Aβ25-35 into the lateral cerebral ventricle. The cognitive ability of the AD model mice was detected by the water maze test and the shuttle box test. The activities of acetylcholinesterase （ACHE） and choline acetyltransferase （CHAT） in the brains were examined by the colorimetric method. The acetylcholine （ACh） content was examined by the alkaline hydroxylamine colorimetric method. Results The Chinese herbal medicine FZS not only effectively improved the learning and memory abilities of the AD model mice in the water maze and step-through tests, but also ameliorated the declined ChAT activity and Ach content in the brain and decreased the elevated AchE activity. The FZS was dose-dependently effective in the therapy of AD in mice ranging from 1.25 to 10 g · kg^-1, however it showed optimal therapeutic effect at the dosage of 2. 5 g · kg^-1. Conclusion The FZS compounds regulate and ameliorate the cholinergic functions of the ADmodel mice, thereby achieve its therapeutic effect.

关 键 词：复智散 阿尔采末病 胆碱乙酰基转移酶 乙酰胆碱酯酶 乙酰胆碱 认知功能 

分 类 号：R-332[医药卫生] R282.71