Transmission electron microscopy and atomic force microscopy characterization of nickel deposition on bacterial cells  

作　　者：WANG Jing HE ShiYing XU LiNa GU Ning 

机构地区：[1]Jiangsu Laboratory for Biomaterials and Devices, National Laboratory of Molecular and Biomolecular Electronics, Department ofBiological Science and Medical Engineering, Southeast University, Nanjing 210096, China

出　　处：《Chinese Science Bulletin》2007年第21期2919-2924,共6页

基　　金：Supported by the National Natural Science Foundation of China (Grant Nos. 60171005, 60371027, 60121101, 20573019 and 90406023);Open Project Foun-dation of Laboratory of Solid State Microstructures of Nanjing University

摘　　要：Recently bacterial cells have become attractive biological templates for the fabrication of metal nano- structures or nanomaterials due to their inherent small size, various standard geometrical shapes and abundant source. In this paper, nickel-coated bacterial cells (gram-negative bacteria of Escherichia coli) were fabricated via electroless chemical plating. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) characterization results reveal evident morphological difference between bacterial cells before and after deposition with nickel. The bare cells with smooth surface presented transverse outspreading effect at mica surface. Great changes took place in surface roughness for those bacterial cells after metallization. A large number of nickel nanoparticles were observed to be equably distributed at bacterial surface after activation and subsequent metallization. Furthermore, ultra thin section analytic results validated the presence and uniformity of thin nickel coating at bacte- rial surface after metallization.Recently bacterial cells have become attractive biological templates for the fabrication of metal nanostructures or nanomaterials due to their inherent small size, various standard geometrical shapes and abundant source. In this paper, nickel-coated bacterial cells （gram-negative bacteria of Escherichia coll were fabricated via electroless chemical plating. Atomic force microscopy （AFM） and transmission electron microscopy （TEM） characterization results reveal evident morphological difference between bacterial cells before and after deposition with nickel. The bare cells with smooth surface presented transverse outspreading effect at mica surface. Great changes took place in surface roughness for those bacterial cells after metallization. A large number of nickel nanoparticles were observed to be equably distributed at bacterial surface after activation and subsequent metallizaUon. Furthermore, ultra thin section analytic results validated the presence and uniformity of thin nickel coating at bacterial surface after metallization.

关 键 词：原子粒显微镜 电子显微镜 金属化 细菌细胞 

分 类 号：Q2[生物学—细胞生物学]