Genistein抑制鸟嘌呤氧化损伤的CGC/MS研究  

Inhibitive Effect of Genistein on the Oxidative Damage to Guanine Studied by CGC/MS

作　　者：宋元宗[1] 庄海旗[2] 张海涛[1] 祝其锋[1] 莫丽儿[2] 

机构地区：[1]广东医学院医用生化研究所,湛江524023 [2]广东医学院医用化学教研室,湛江524023

出　　处：《肿瘤防治研究》1997年第3期142-145,共4页Cancer Research on Prevention and Treatment

基　　金：广东省重点学科资助

摘　　要：为了探讨genistein抗肿瘤与抗氧化之间的关系，用Fenton反应（H2O2＋Fe2+→·OH＋OH－＋Fe3+）造成鸟嘌呤的氧化损伤，用毛细管柱气相色谱／质谱分析法（CGC／MS）研究了genistein对这一过程的影响。结果表明genstein具有抑制8-经基鸟嘌呤生成的作用，60μmol·L－1genistein可使8-经基鸟嘌呤的生成量降低50％，提示genistein的抗肿瘤效果可能与其保护遗传物质的氧化损伤有关，这种作用可能是genistein通过清除H2O2造成的。Genistein is a isoflavone with antioxidant property and antitumor activity. 8hydroxyguanine is a sensitive marker for the oxidative damage to DNA, and a wellestabilished biomarker relevant to carcinogenesis and aging as well. To study the relationship between the antioxidant property of genistein and its antitumor activity, in this paper, the effect of genistein on the damage to guanine induced by Fenton reaction (H,O, + Fe2+ →. OH +OH- + Fe3+ ) was studied using capillary gas chromatography/mass spectrometry. The results show that genistein can inhibit the formation of 8-hydroxyguanine potently, and at a concentration of 60μmol·L-1genistein can reduce the production of 8-hydroxyguanine to 50% of that in control,which suggest that the inhibitive effect of genistein on H2O2-induced DNA damagemaybe implicated in its antitumor activity. Based on this paper and other studies, it is suggested that this inhibitive effect of genistein results from the scavenging of H2O2 by genistein.

关 键 词：气相色谱 质谱分析 鸟嘌呤 氧化损伤 GENISTEIN 

分 类 号：R979.1[医药卫生—药品] R284.1[医药卫生—药学]