白菜春化相关基因BcFLC的克隆及表达研究  

Cloning and Expression Analysis of Vernalization-related Gene BcFLC in Brassica campestris

作　　者：黄细松[1] 李晋豫[1] 余小林[1] 孙保娟[1] 曹家树[1] 

机构地区：[1]浙江大学蔬菜研究所细胞与分子生物学实验室,杭州310029

出　　处：《园艺学报》2007年第5期1169-1176,共8页Acta Horticulturae Sinica

基　　金：浙江省重大科技项目(2005C12019/02);浙江省自然科学基金项目(Y304164)

摘　　要：运用同源基因克隆法从‘上海青’白菜(Brassica campestris ssp.chinensis var.communis‘Shanghai Qing’)和‘四九’菜薹(var.parachinensis‘Sijiu’)叶片中分离到FLC的同源基因BcFLC的编码序列、启动子以及内含子1序列;序列比对发现,二者编码的核苷酸序列几乎完全一致,而且启动子及内含子1序列也无实质性差异;对‘上海青’进行人工低温春化处理,BcFLC-1基因在‘上海青’茎尖的表达随着低温处理时间的延长逐渐减弱,低温处理20 d时BcFLC-1基因的表达已经非常弱,低温处理30 d时,表达已检测不到,花芽分化石蜡切片也表明,低温处理20 d,茎尖已开始向生殖状态过渡。BcFLC-2基因在‘四九’菜薹未现蕾之前的叶片中有强表达,而现蕾之后叶片中则检测不到。　In order to investigate the flowering repressor gene FLC in Chinese cabbage-pak-choi, by homologous cloning and RACE, vernalization-related gene BcFLC-1 and BcFLC-2 were respectively isolated from Brassica campestris ssp. chinensis var. communis‘Shanghai Qing’and Brassica campestris ssp. chinensis var. parachinensis‘Sijiu’.The results of alignment indicated that there was no difference between the coding sequences（CDS）of BcFLC-1 and BcFLC-2, and the nucleotide sequence of promoter and intron 1 of them were of no substantial difference. RT-PCR analysis showed that the expression of BcFLC-1 in the shoot apical meristem of‘Shanghai Qing’was reduced gradually by the time of artificially vernalization at 4℃,and it got a very low level after 20 days and was undetectable after 30 days. The paraffin section also proved that 20 days cold treatment was enough for the apical meristem transforming to reproductive development. BcFLC-2 was strongly expressed in the leaf of flowering Chinese cabbage‘Sijiu’ before floral buds appear;however, the expression of BcFLC-2 was undetectable after floral buds＇appearance.

关 键 词：白菜 菜薹 春化 FLC基因 

分 类 号：S634.3[农业科学—蔬菜学]