胃癌组织中胸苷酸合成酶、胸苷磷酸化酶mRNA表达及其临床意义  

Expression of thymidylate synthase and thymidine phosphorylase in gastric cancer and their correlation with prognosis

作　　者：华东[1] 黄朝晖[1] 邓建忠[1] 茆勇[1] 

机构地区：[1]苏州大学附属第四医院肿瘤内科中心实验室,江苏无锡214062

出　　处：《中国癌症杂志》2007年第10期766-769,共4页China Oncology

基　　金：无锡市科技局科研项目(CS055012)

摘　　要：背景与目的:化学治疗在胃癌治疗中扮演了重要的作用。通过肿瘤组织中某些基因表达的水平来选择化疗药物已成为今后化疗的方向。胸苷酸合成酶(TS)、胸苷磷酸化酶(TP)是氟尿嘧啶(5-FU)体内的关键代谢酶,其表达水平影响恶性肿瘤患者接受5-FU化疗后的预后。本研究探讨胃癌组织中TS、TP mRNA表达水平与预后的关系。方法:采用实时定量RT-PCR技术检测51例胃腺癌组织TS、TP mRNA表达水平。结果:胃癌组织TS、TP mRNA表达水平的中位数分别为0.94和21.20,TS高表达组和低表达组之间无瘤生存期和总生存期差异有显著性(P<0.05),TP高表达组和低表达组之间总生存期差异有显著性(P<0.05),但无瘤生存期之间差异无显著性(P>0.05)。TS、TP mRNA表达与年龄、性别、淋巴结转移、组织学分级及临床分期均无相关性(P>0.05)。结论:检测TS、TP mRNA表达水平对接受5-FU为基础治疗方案的胃癌患者的预后有很好的预测价值。Background and purpose： Chemotherapy plays an important role in the treatment of gastric cancer. Ii is hecoming a direction that the choice of chemotherapeutic agent for the clinie will be based on the levels of some relative gene expressions in the tumor tissue. As the key enzymes of fluoropyrimidines, the expression levels of thymidylate synthase （TS） and thymidine phosphorylase （TP） may correlate with the response of tamor to chemotherapy. This study was to investigate the expression of TS and TP mRNA in gastric cancer tissues and their correlation with prognosis. Methods： The expression levels of TS, TP mRNA in 51 gastric adenocarcinoma tissues were detected by real-time quantitative reverse transeription polymerase chain reaction （RT-PCR）. Results： The median expression levels of TS, TP mRNA were 0.94 and 21.20. respectively. There was significant difference in terms of disease-free and overall survival between the gastric caneer patients with high and low TS mRNA expression level （ P 〈0.05） ; no difference in disease-free survival existed between the gastric cancer patients with high and low TP mRNA expressiun level（ P 〉0.05） but not overall survival （ P 〈0.05）. The expression levels of TS , TP mHNA were not correlated with age, gender, histological grade, clinical stage and lymph node metastasis（ P〉 0.05）. Conclusions： The expression levels of TS, TP mRNA may serve as prognostie markers for gastric cancer patients treated with FU-based adjuvant chemotherapy.

关 键 词：胃肿瘤 胸苷酸合成酶 胸苷磷酸化酶 预后 氟尿嘧啶 

分 类 号：R735.2[医药卫生—肿瘤] R730.7[医药卫生—临床医学]