准分子激光原位角膜磨镶术前后泪液中神经生长因子水平与泪膜稳定性的相关性分析  

The relationship between the levels of nerve growth factor in tears and tear film stabilitv after LASIK

作　　者：曹雨金[1] 唐仁泓[1] 

机构地区：[1]长沙中南大学湘雅三医院,长沙410013

出　　处：《中国实用眼科杂志》2007年第9期992-994,共3页Chinese Journal of Practical Ophthalmology

摘　　要：目的 探讨内源性神经生长因子（nerve growth factor,NGF）在准分子激光原位角膜磨镶术（1aser in situ keratomileusis,LASIK）后干眼中的作用机制。方法 LASIK术前及术后1d、2d、10d及30d在17名患者（34眼）下睑结膜囊内抽取泪液标本,采用双抗体夹心酶联免疫吸附分析法检测泪液中NGF含量,同时行泪膜稳定性的检查,并对两者行相关性分析。结果 NGF含量在术前平均值为（21.964±8.22）μg/ml,术后ld,2d,10d含量分别为（12.764±3.55）μg/ml、（11.01±4.66）μg/m1、（13.554±4.93）μg/ml,与术前比较差异有显著性（P〈0.05）;术后30d含量（19.164±8.79）μg/m1,与术前比较差异无显著性（P〉0.05）。术前汨膜破裂时间（breaking-uptime,BUT）均值为（12.624±1.99）s,术后1 d、2d、10d和30d分别为（4.684±1.77）s、（4.84±1.92）s、（7.36±1.53）s和（8.824-3.08）s,与术前比较差异有显著性（P〈0.05）：术后1d与2d比较差异无显著性（P〉0.05）,术后1d（2d）、10d、30d比较差异均有显著性（P〈0.05）。泪液中NGF含量与泪膜稳定性存在正相关（r=0.484,P〈0.01）。结论 LASIK术后早期泪液NGF的含量存在一个由降低到正常的动态变化过程,干眼主要与泪膜稳定性下降有关,NGF含量变化是泪膜稳定性下降的相关因素之一。Objective To study the mechanism of endogenous nerve growth factor in the dry eye after LASIK. Methods Nerve growth factor（NGF）levels in the tears of 34 eyes before and aner LASIK were studied with a monoclonal antibody based ELISA before LASIK and 1 day,2days,10days,and 30days after LASIK.Tear film stability was examined during the same visits.And then study the relationship between the levels of NGF in tears and tear film stability in the eyes of pre and postoperative LASIK patients.Results The NGF mean value before LASIK was（21.96 ± 8.22）μg/ml and mean values at 1 day,2 days,10 days,and 30 days after LASIK were （12.76 ±3.55）μg/ml,（11.01 ±4.66）μg/ml,（13.554 ± 4.93）μg/ml,（19.164 ± 8.79）μg/ml, respectively.The NGF levels in tears at the first three visits post-operaion were SigniflCantlyfour visits after surgery was significantly different from the preoperative level（P〈0.05）.There was a positive correlation between tear film content and changes in tear film stability after LASIK（r=0.484,P〈0.01）.Conclusion The decrease of BUT is the cause of dry eye and the changes in tear film stability and the onset of dry eye after LASIK are related to the level of NGF.

关 键 词：准分子激光原位角膜磨镶术 神经生长因子 泪膜稳定性 干眼 

分 类 号：R779.63[医药卫生—眼科]