大鼠局灶性脑缺血再灌注后凋亡诱导因子的表达及其与神经细胞凋亡的关系  

The expression of apoptosis inducing factor after focal cerebral ischemia and reperfusion in rats and its relation to nerve cell apoptosis

作　　者：冯建利[1] 姚博[1] 闫鹏[1] 杜怡峰[1] 

机构地区：[1]山东大学山东省立医院神经内科,山东济南250021

出　　处：《中国老年学杂志》2007年第20期1955-1957,共3页Chinese Journal of Gerontology

基　　金：山东省卫生厅科技发展项目(2001CA1CBB1)

摘　　要：目的观察大鼠脑缺血再灌注(I/R)后凋亡诱导因子(AIF)的表达,探讨I/R脑区AIF变化与神经细胞凋亡的关系。方法应用线栓法建立Wistar大鼠大脑中动脉阻塞(MCAO)再灌注模型,TTC染色观察梗死灶的形成,应用原位末端标记(TUNEL)和免疫组化技术分别观察大鼠假手术组、MCAO90min再灌注0、3、6、12、24、48、72h时额顶叶皮层缺血脑区AIF表达及神经细胞凋亡程度的变化。结果脑I/R24h,TTC染色见明显的梗死灶形成。除缺血90min凋亡细胞数量与假手术组比较无统计学意义外,其余各指标与假手术组相比均有统计学意义(P<0.01),除6与12h以及24与48hAIF含量的差异无统计学意义,24与48h凋亡细胞数量的差异无统计学意义外,其余各组间差异均有统计学意义(P<0.05)。结论I/R可诱导AIF阳性细胞表达增加,并引起神经细胞凋亡。Objective To observe the expression of apoptosis inducing factor （AIF） after focal cerebral ischemia and reperfusion （I/R） in rats and explore relationship between the changes in AIF and nerve cell apoptosis. Methods Wistar middle cerebral artery occlusion （MCAO） reperfusion rat model was established by thread ligation. The infarction focus formation was observed by TIC staining. AIF ex- pression in ischemia region of cortex of parietal lobe and nerve cell apoptosis degree in sham operation group, MCAO 90 min and reperfusion 0, 3, 6, 12, 24, 48 and 72 h were observed by TUNEL and immunohistochemistry technique. Results The infarction focus was obviously observed by TIC staining in I/R 24 h. Except the apoptosis cell number, the other indexes in reperfusion groups had significant difference form those in sham operation group （P 〈0. 01 ）. There were no significant difference in AIF contents between I/R 6 h and 12 h, between I/ R 24 h and 48 h, no difference in apoptosis cell number between I/R 24 h and 48 h, but those among the other groups （P 〈0.05）. Conclusions I/R could induce the increased AIF positive cell expression and nerve cell apoptosis.

关 键 词：脑缺血 细胞凋亡 凋亡诱导因子 

分 类 号：R743.33[医药卫生—神经病学与精神病学] R361.3[医药卫生—临床医学]