脑血管穿破法建立大鼠蛛网膜下腔出血模型  

Establishment of experimental subarachnoid hemorrhage model with endo-cerebrovascular perforation

作　　者：郑杰胜[1] 马跃辉[1] 詹仁雅[1] 周永庆[1] 

机构地区：[1]浙江大学医学院附属第一医院神经外科,杭州310003

出　　处：《中华急诊医学杂志》2007年第10期1050-1053,共4页Chinese Journal of Emergency Medicine

基　　金：浙江省科技攻关项目(2007C30002)

摘　　要：目的采用脑底动脉环穿破法,制作一种稳定可靠且不需开颅的大鼠蛛网膜下腔出血(SAH)模型。方法大鼠麻醉后暴露右侧颈总动脉主干、颈外动脉及颈内动脉。从颈外动脉插入3 -0单丝尼龙线至颈内动脉,当尼龙线头部距颈总动脉分又约18～19 mm时,感到阻力,略用力再伸入尼龙线2 mm,然后完全退出尼龙线,结扎颈外动脉,缝合颈部皮肤。造模后2 h和48 h,观察SAH的程度和范围;造模后48 h测定基底动脉直径。结果造模后2 h蛛网膜下腔有明显出血,范围累及整个脑底动脉环;造模后48 h,可见陈旧性血块附着在脑底动脉环周围。造模后48 h,基底动脉出现痉挛。结论采用脑底动脉环穿破法能成功建立大鼠SAH模型。Objective To establish an experimental subarachnoid hemorrhage （SAH） model with endocerebrovascular perforation. Method The right external carotid artery of SD rats were isolated, leaving a stump of approximately 3 to 4 mm. A-3-0 monofilament nylon suture was inserted up through the stump of external carotid artery to the internal carotid artery for about 18 - 19 mm. A small resistance was usually felt, and the suture was then advanced 2 mm further and the suture was immediately withdrawn. Two hours or two days after SAH induction, SAH extension was observed. Two days after SAH induction, the diameter of the basilar artery was measured. Results SAH extends from the ipsilateral artery to the contralateral artery after SAH induction. The diameters of basilar arteries in SAH animals were smaller than those of control rats, indicating the present of cerebrovascular spasm in SAIl animals. Conclusions The endo-cerebrovascular perforation technique for establishing a non-craniotomy SAH model is reliable.

关 键 词：蛛网膜下腔出血 动物模型 血管痉挛 

分 类 号：R743.3[医药卫生—神经病学与精神病学]