雄黄对荷人卵巢癌裸鼠移植瘤细胞凋亡的基础研究  

The effect of realgar on apoqtosis of transplanted ovarian SKOV3 carcinoma cells in nude mice

作　　者：陈文雪[1] 张峰[2] 杨会钗[3] 刘爽[3] 郭霞[4] 李晶[4] 

机构地区：[1]河北医科大学第四医院妇产科,石家庄050011 [2]河北医科大学第一医院普外科,石家庄050000 [3]河北医科大学第四医院病理科,石家庄050011 [4]河北医科大学第四医院中医科,石家庄050011

出　　处：《肿瘤》2007年第10期787-790,共4页Tumor

基　　金：河北省科技厅基金资助项目(编号:05276101D-58;06276102D-04)

摘　　要：目的:观察中药雄黄诱导卵巢癌细胞凋亡及血管生成的作用。方法:应用流式细胞术、荧光显微镜、透射电子显微镜和免疫组织化学等技术,以细胞凋亡和VEGF为主要观测指标,观察中药雄黄对荷人卵巢癌裸鼠移植瘤抗肿瘤作用。结果:雄黄可诱导荷人卵巢癌裸鼠移植瘤细胞凋亡,抑制荷瘤裸鼠肿瘤的生长,阻止血管生成抑制细胞DNA的合成,并可延长小鼠存活时间。结论:雄黄可通过诱导卵巢癌细胞凋亡,阻止血管生成,抑制细胞DNA的合成等多种途径发挥抗肿瘤作用,具有广阔的应用前景。Objective：To observe the effect of traditional Chinese medicine, realgar, on apoptosis and angiogenesis of ovarian carcinoma cells. Methods： The antl-tumor effects of realgar were observed in nude mice bearing ovarian transplanted carcinoma. Apoptosis and the expression of vascular endothelial growth factor were determined by flow cytometry, fluorescence microscopy, transmission electron microscopy, and immunohistochemical methods. Results： Realgar induced apoptosis and inhibited the growth of ovarian transplanted tumors in nude mice. Realgar also blocked the angiogenesis, inhibited the DNA synthesis, and prolonged the survival time of nude mice. Conclusion：Realgar exerted its anti-tumor effect through multiple pathways such as inducing apoptosis, blocking angiogenesis, inhibiting DNA synthesis and had a wide foreground for application.

关 键 词：卵巢肿瘤 雄黄 抗肿瘤药(中药) 细胞凋亡 

分 类 号：R737.31[医药卫生—肿瘤]