宫颈鳞癌和癌前病变中eIF-4E表达及其与HPV16/18感染的关系  

Expression of eIF-4E in cervical squamous cell carcinoma and precancerous lesions and its correlation with HPV16/18 infection

作　　者：潘保见[1] 陈嘉薇[1] 祝峰[1] 

机构地区：[1]上海交通大学附属第一人民医院病理科,200080

出　　处：《临床与实验病理学杂志》2007年第5期528-531,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨真核翻译启始因子eIF-4E在宫颈鳞癌和癌前病变中的表达规律及其与人乳头瘤病毒(HPV)感染之间的关系。方法采用免疫组化ABC法和原位杂交技术检测eIF-4E蛋白和HPV16/18DNA在10例正常宫颈鳞状上皮,29例低级别上皮内瘤变(CIN1级),31例高级别上皮内瘤变(CIN2、3级)和31例宫颈鳞癌中的表达。结果eIF-4E在正常宫颈鳞状上皮中呈阴性表达,随着上皮病变级别升高,表达逐渐增强:低级别<高级别上皮内瘤变<宫颈鳞癌(P<0.05),且在宫颈鳞癌中表达更强;HPV16/18DNA在四组中的阳性表达率,除高级别上皮内瘤变和宫颈鳞癌两组之间没有差别(均为96.8%)以外,其余各组之间差异均有显著性(P<0.01);在低级别、高级别上皮内瘤变和宫颈鳞癌三组中eIF-4E蛋白表达和HPV感染均呈明显正相关(P<0.01)。结论宫颈鳞癌的发生与eIF-4E蛋白表达及HPV16/18感染密切相关;二者在宫颈鳞癌的发病机制中可能起着协同作用。Purpose To study the expression of eIF-4E in cervical squamous cell carcinoma （SCC） and precancerous lesions and to analyze the relationship between the expression of eIF-4E and the infection of human papilloma virus （HPV）. Methods The expression of eIF-4E protein and HPV16/18 DNA was determined by immunohistochemistry and in situ hybridization in 10 cases of normal cervical squamous 29 cases of low-grade intraepithelial neoplasia, 31 cases of high-grade intraepithelial neoplasia and 31 cases of cervical SCC. Results eIF-4E expression was not detected in normal cervical squamous epithelia, gradually increased with grading of epithelial lesions in the order of low-grade 〈 high-grade 〈 SCC （P 〈 0.05 ）, and in particular,was much stronger in cases of SCC. The positive rate of HPV 16/18 DNA among the groups of normal cervical squamous epithelia, low grade, high-grade intraepithelial neopiasia and SCC was significantly different （ P 〈 0. 01 ）, except two groups of high-grade intraepithelial neoplasia and SCC in which the positive rate was both 96.8%. In low-grade high-grade intraepithelial neoplasia and SCC, eIF-4E expression had obviously positive correlation with HPV16/18DNA infection （ P 〈 0. 01 ）. Conclusions The occurrence of SCC may be intimately related to the abnormal expression of eIF-4E and the infection of HPV16/18. Both may cooperate in the pathogenesis of cervical SCC.

关 键 词：子宫颈肿瘤 鳞癌 EIF4E HPV16/18感染 

分 类 号：R737.33[医药卫生—肿瘤]