乳腺肿瘤冷冻切片诊断中快速EliVision技术的价值  

Evaluation of rapid immunohistochemical staining technique in intraoperative frozen section diagnosis of breast tumors

作　　者：何沁[1] 徐华[1] 邵牧民[1] 余学问[1] 

机构地区：[1]广州中医药大学深圳附属医院病理科,深圳518033

出　　处：《临床与实验病理学杂志》2007年第5期553-556,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨快速免疫组化技术(immunohistochemistry,IHC)在乳腺肿瘤术中冷冻病理诊断中的应用价值。方法采用EliVision快速IHC技术检测乳腺肿瘤冷冻切片中常用抗体(SMA、CK5/6、p63、c-erbB-2、CD34、CD117、ER、PR)的表达情况,以相应病例冷冻切片的EliVision常规IHC法以及冰余组织EliVision常规免疫组化法作对照。结果EliVision快速IHC免疫组化法在30min内完成检测,7种抗体显示明确而定位可靠的阳性表达结果(c-erbB-2仅在常规IHC中表达),阳性率及阳性强度高于相应病例冷冻切片的EliVision常规IHC法、接近于冰余组织EliVision常规IHC法。结论EliVision快速IHC技术可以作为乳腺肿瘤术中冷冻切片病理诊断中有价值的辅助手段。Purpose To evaluate the significance of the rapid immunohistochemical （IHC） staining technique in intraoperative frozen section diagnosis of breast tumors, Methods Eight primary antibodies （including SMA, CK5/6, p63, c-erbB-2, CD34, CDll7, ER and PR） were tested using the rapid EliVision plus immunostaining on frozen sections of breast tumors, The results of the modified rapid immunostaining on frozen sections were compared with the results of the routine EliVision IHC method, Results The rapid immunostaining method allowed the detection of the breast tumor antigens on frozen sections in less than 30 min. This rapid procedure produced strong and specific staining that was similar to the routine IHC procedures and was clearly distinguishable from the weak background staining with 7 of 8 primary antibodies applied, c-erbB-2 was positive only in routine EliVision IHC procedures, Conclusion The rapid EliVision plus immunostaining method could represent a useful tool in frozen section diagnosis of breast tumors.

关 键 词：乳腺肿瘤 免疫组织化学 冷冻切片 EliVision法 

分 类 号：R737.9[医药卫生—肿瘤] R446.8[医药卫生—临床医学]