Space-flight Mutation of Streptomyces gilvosporeus for Enhancing Natamycin Production  

作　　者：梁景乐 林建平 徐志南 苏薇 岑沛霖 

机构地区：[1]Institute of Bioengineering, College of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China [2]Shandong Lukang Pharmaceutical Co., Jining 272021, China

出　　处：《Chinese Journal of Chemical Engineering》2007年第5期720-724,共5页中国化学工程学报（英文版）

摘　　要：Mutants of the strain producing natamycin, Streptomyces gilvosporeus, were obtained after space-flight mutation. With respect to the sand spores and slant spores, the mutation ratios were up to 67.6% and 78.3% and the survival ratio was 43.1% and 3.0%, respectively. An improved mutant producing natamycin, S. gilvosporeus LK-45, was screened, which showed natamycin productivity of 1420mg·L^-1. A mutant resistant to 2-deoxy glucose, S.gilvosporeus LK-119, was further obtained using a＇rational screening procedure. The natamycin productivity of 1940mg·L^-1 was achieved when glucose was used as the carbon source.生产 natamycin 的紧张的异种， Streptomyces gilvosporeus，在空间飞行以后被获得变化。关于沙孢子和观点孢子，变化比率直到 67.6% 和 78.3% ，幸存比率分别地是 43.1% 和 3.0% 。生产 natamycin 的改进异种， S。gilvosporeus LK-45，被屏蔽，它显示出 1420mg · L 的 natamycin 生产率 ? 1。对 2-deoxy 葡萄糖抵抗的异种， S。gilvosporeus LK-119，进一步用一个合理屏蔽过程被获得。1940mg · L 的 natamycin 生产率 ? 当葡萄糖被用作碳来源时， 1 被完成。

关 键 词：space-flight mutation Streptomyces gilvosporeus NATAMYCIN rational screening 2-deoxy glucose 

分 类 号：TQ46[化学工程—制药化工]